Sensitivity of Human Mastadenovirus, the Causal Agent of Pharyngoconjunctival Fever, Epidemic Keratoconjunctivitis, and Hemorrhagic Cystitis in Immunocompromised Individuals, to Brincidofovir

• Published in: Microbiology spectrum Vol. 10; no. 1; p. e0156921
• Main Authors: Hanaoka, Nozomu, Hazama, Masatoshi, Fukushima, Koji, Fujimoto, Tsuguto
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 23.02.2022
• Subjects: Adenoviridae Infections - immunology; Adenoviridae Infections - virology; Adenovirus Infections, Human - immunology; Adenovirus Infections, Human - virology; brincidofovir; Cystitis; Cytosine - analogs & derivatives; Cytosine - pharmacology; half-maximal inhibitory concentration; human mastadenovirus; Humans; Immunocompromised Host; Keratoconjunctivitis - immunology; Keratoconjunctivitis - virology; Mastadenovirus - classification; Mastadenovirus - drug effects; Mastadenovirus - genetics; Mastadenovirus - physiology; novel type; Observation; Organophosphonates - pharmacology; Virology; human mastadenovirus; half-maximal inhibitory concentration; novel type; brincidofovir
• ISSN: 2165-0497; 2165-0497
• DOI: 10.1128/spectrum.01569-21

We investigated the IC 50 values of BCV for novel HAdV types and found that the epidemic keratoconjunctivitis-associated HAdV-D54 prevalent in the Asian region was the most susceptible. In addition, the mean overall IC 50 value of BCV was lower than that of CDV, indicating that BCV is effective against HAdVs. Human mastadenovirus (HAdV), a linear double-stranded DNA (dsDNA) virus, is the causal agent of several diseases, including pharyngoconjunctival fever, epidemic keratoconjunctivitis, and hemorrhagic cystitis, in immunocompromised individuals. There are more than 100 reported types of adenoviruses, but the pathogenicity of many HAdVs remains unknown. Brincidofovir (BCV) is a hexadecyloxypropyl lipid conjugate of cidofovir (CDV) that is active against dsDNA viruses. Clinical effectiveness of BCV against certain HAdV species has been reported; however, its activity against novel HAdV types remains unknown. We investigated the half-maximal inhibitory concentration (IC 50 ) values of BCV for novel HAdV types and found that the epidemic keratoconjunctivitis-associated HAdV-D54 prevalent in the Asian region was the most susceptible. The mean overall IC 50 value of BCV was lower than that of CDV, indicating that BCV is effective against HAdVs, including the novel types. IMPORTANCE We investigated the IC 50 values of BCV for novel HAdV types and found that the epidemic keratoconjunctivitis-associated HAdV-D54 prevalent in the Asian region was the most susceptible. In addition, the mean overall IC 50 value of BCV was lower than that of CDV, indicating that BCV is effective against HAdVs.