FUNCTIONAL MODULATION OF ESCHERICHIA COLI RNA POLYMERASE

• Published in: Annual review of microbiology Vol. 54; no. 1; pp. 499 - 518
• Main Author: Ishihama, Akira
• Format: Journal Article
• Language: English
• Published: Palo Alto, CA 94303-0139 Annual Reviews 01.01.2000; 4139 El Camino Way, P.O. Box 10139 Annual Reviews, Inc; USA
• Subjects: Bacteria; Biological and medical sciences; DNA-Directed RNA Polymerases - metabolism; E coli; Escherichia coli; Escherichia coli - enzymology; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Microbiology; Protein Binding; Protein Subunits; Ribonucleic acid; RNA; sigma factor; Sigma Factor - antagonists & inhibitors; Sigma Factor - metabolism; stationary phase; Techniques used in virology; transcription apparatus; transcription factor; Transcription Factors - metabolism; Transcription, Genetic; Virology; Nucleotidyltransferases; Transcription; Enzyme; Transferases; Escherichia coli; Initiation factor σ; Bacteria; Review; Transcription factor; DNA-directed RNA polymerase; Enterobacteriaceae
• ISSN: 0066-4227; 1545-3251
• DOI: 10.1146/annurev.micro.54.1.499

The promoter recognition specificity of Escherichia coli RNA polymerase is modulated by replacement of the σ subunit in the first step and by interaction with transcription factors in the second step. The overall differentiated state of ∼2000 molecules of the RNA polymerase in a single cell can be estimated after measurement of both the intracellular concentrations and the RNA polymerase-binding affinities for all seven species of the σ subunit and 100-150 transcription factors. The anticipated impact from this line of systematic approach is that the prediction of the expression hierarchy of ∼4000 genes on the E. coli genome can be estimated.