Rubisco Function, Evolution, and Engineering

• Published in: Annual review of biochemistry Vol. 92; pp. 385 - 410
• Main Authors: Prywes, Noam, Phillips, Naiya R, Tuck, Owen T, Valentin-Alvarado, Luis E, Savage, David F
• Format: Journal Article
• Language: English
• Published: United States Annual Reviews 20.06.2023
• Subjects: Calvin cycle; carbon fixation; carboxylases; enzyme engineering; rubisco; enzyme engineering; carboxylases; rubisco; Calvin cycle; carbon fixation
• ISSN: 0066-4154; 1545-4509
• DOI: 10.1146/annurev-biochem-040320-101244

Carbon fixation is the process by which CO 2 is converted from a gas into biomass. The Calvin-Benson-Bassham cycle (CBB) is the dominant carbon-consuming pathway on Earth, driving >99.5% of the ∼120 billion tons of carbon that are converted to sugar by plants, algae, and cyanobacteria. The carboxylase enzyme in the CBB, ribulose-1,5-bisphosphate carboxylase oxygenase (rubisco), fixes one CO 2 molecule per turn of the cycle into bioavailable sugars. Despite being critical to the assimilation of carbon, rubisco's kinetic rate is not very fast, limiting flux through the pathway. This bottleneck presents a paradox: Why has rubisco not evolved to be a better catalyst? Many hypothesize that the catalytic mechanism of rubisco is subject to one or more trade-offs and that rubisco variants have been optimized for their native physiological environment. Here, we review the evolution and biochemistry of rubisco through the lens of structure and mechanism in order to understand what trade-offs limit its improvement. We also review the many attempts to improve rubisco itself and thereby promote plant growth.