Enhanced anaerobic dechlorination of polychlorinated biphenyl in sediments by bioanode stimulation

• Published in: Environmental pollution (1987) Vol. 211; pp. 81 - 89
• Main Authors: Yu, Hui, Feng, Chunhua, Liu, Xiaoping, Yi, Xiaoyun, Ren, Yuan, Wei, Chaohai
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.04.2016
• Subjects: Acetates; Actinobacteria; Alcanivorax; Anaerobiosis; Bacteria; Bioanode stimulation; bioanodes; Biodegradation, Environmental; Bioelectrochemical system; Bioreactors; bioremediation; China; Chloroflexi; Comamonas; Concentration (composition); cost effectiveness; Dechlorination; Dysgonomonas; Electric field; electrochemistry; Electrodes; electronic wastes; Environmental Pollutants - analysis; Environmental Pollutants - metabolism; Environmental Restoration and Remediation - methods; Geobacter; Geologic Sediments - chemistry; Halogenation; High-throughput sequencing; Hydrogenophaga; Ignavibacterium; mercurous chloride; PCB remediation; Polychlorinated biphenyls; Polychlorinated Biphenyls - analysis; Polychlorinated Biphenyls - metabolism; recycling; Sediments; Stimulation; Transformations; Vibrio; China, People's Rep; China; PCB remediation; Electric field; High-throughput sequencing; Bioelectrochemical system; Bioanode stimulation
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2015.12.039

The application of a low-voltage electric field as an electron donor or acceptor to promote the bioremediation of chlorinated organic compounds represents a promising technology meeting the demand of developing an efficient and cost-effective strategy for in situ treatment of PCB-contaminated sediments. Here, we reported that bioanode stimulation with an anodic potential markedly enhanced dechlorination of 2,3,4,5-tetrachlorobiphenyl (PCB 61) contained in the sediment at an electronic waste recycling site of Qingyuan, Guangdong, China. The 110-day incubation of the bioanode with a potential poised at 0.2 V relative to saturated calomel electrode enabled 58% transformation of the total PCB 61 at the initial concentration of 100 μmol kg−1, while only 23% was reduced in the open-circuit reference experiment. The introduction of acetate to the bioelectrochemical reactor (BER) further improved PCB 61 transformation to 82%. Analysis of the bacterial composition showed significant community shifts in response to variations in treatment. At phylum level, the bioanode stimulation resulted in substantially increased abundance of Actinobacteria, Bacteroidetes, and Chloroflexi either capable of PCB dechlorination, or detected in the PCB-contaminated environment. At genus level, the BER contained two types of microorganisms: electrochemically active bacteria (EAB) represented by Geobacter, Ignavibacterium, and Dysgonomonas, and dechlorinating bacteria including Hydrogenophaga, Alcanivorax, Sedimentibacter, Dehalogenimonas, Comamonas and Vibrio. These results suggest that the presence of EAB can promote the population of dechlorinating bacteria which are responsible for PCB 61 transformation. •A bioelectrochemical reactor (BER) was constructed for anaerobic PCB dechlorination.•Bioanode stimulation substantially enhanced dechlorination of PCB 61.•Electrochemically active bacteria and dechlorinating bacteria coexisted in the BER. Bioanode stimulation at a low applied potential can promote anaerobic dechlorination of a model PCB congener (i.e., PCB 61) that was spiked to the sediment taken from a local electronic waste recycling site.