IL-1 and its role in rat carrageenan pleurisy

• Published in: Mediators of Inflammation Vol. 1993; no. 1; pp. 33 - 39
• Main Authors: Goodman, R, Mantegna, L R, McAilister, C L, Bruin, E, Dowling, R L, George, H, Feeser, W, Freimark, B, Lischwe, M, Pick, S, Harris, R R, Kerr, J S
• Format: Journal Article
• Language: English
• Published: United States Hindawi Limiteds 1993; Hindawi Publishing Corporation; Wiley
• Subjects: Inflammation; Carrageenan; Cytokines; IL-1; Pleurisy
• ISSN: 0962-9351; 1466-1861
• DOI: 10.1155/S0962935193000043

THE carrageenan pleurisy model, which is characterized by cellular influx and oedema, has been used to examine the effects of anti-inflammatory compounds such as naproxen. Interleukin-lt and β (IL-1) are known to be pro-inflammatory mediators, and their roles in this model are unknown. Intrapleural injection of 1% viscarin carrageenan or saline was administered to male Lewis rats. Four to 24 h later, cell counts, fluid volumes and IL-1β levels (measured by ELISA) were determined in the pleural cavity. Serum corticosterone levels were measured only at 4 h. Significant increases in IL-1β levels precede cell influx suggesting IL-1β plays a role in the maintenance of cell accumulation in the pleural cavity. None of the drugs tested, including the IL-1 receptor antagonist, maintained pleural cell influx and IL-1β levels at control levels. When human IL-1α or β or rat IL-1β were injected individually into the pleural cavity, none of these cytokines were pro-inflammatory, as measured by increased cell influx and fluid extravasation. These results suggest that although IL-1β levels increase in the pleural cavity in response to carrageenan, IL-1 per se is not the initiator of the pro-inflammatory events of cell influx and oedema in this model.