Analyzing ribosome remodeling in health and disease

regulation largely unexplored, in part due to methodological limitations. Indeed, we review evidence demonstrating that commonly used methods, such as transcriptomics, are inadequate because the variability in mRNAs coding for ribosomal proteins (RP) does not necessarily correspond to RP variability...

Full description

Saved in:
Bibliographic Details
Published inarXiv.org
Main Authors Petelski, Aleksandra A, Slavov, Nikolai
Format Paper Journal Article
LanguageEnglish
Published Ithaca Cornell University Library, arXiv.org 02.08.2020
Subjects
Protein synthesis
Proteins
Quantitative Biology - Biomolecules
Quantitative Biology - Molecular Networks
Quantitative Biology - Quantitative Methods
Quantitative Biology - Subcellular Processes
Online AccessGet full text

Cover

More Information
Summary:regulation largely unexplored, in part due to methodological limitations. Indeed, we review evidence demonstrating that commonly used methods, such as transcriptomics, are inadequate because the variability in mRNAs coding for ribosomal proteins (RP) does not necessarily correspond to RP variability. Thus protein remodeling of ribosomes should be investigated by methods that allow direct quantification of RPs, ideally of isolated ribosomes. We review such methods, focusing on mass spectrometry and emphasizing method-specific biases and approaches to control these biases. We argue that using multiple complementary methods can help reduce the danger of interpreting reproducible systematic biases as evidence for ribosome remodeling.
ISSN:2331-8422
DOI:10.48550/arxiv.2007.05839