Lignans from Dysosma versipellis with Inhibitory Effects on Prostate Cancer Cell Lines

• Published in: Journal of natural products (Washington, D.C.) Vol. 70; no. 2; pp. 283 - 286
• Main Authors: Jiang, Ren-Wang, Zhou, Jin-Rong, Hon, Po-Ming, Li, Song-Lin, Zhou, Yan, Li, Ling-Lin, Ye, Wen-Cai, Xu, Hong-Xi, Shaw, Pang-Chui, But, Paul Pui-Hay
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 01.02.2007; Amer Chemical Soc; American Society of Pharmacognosy
• Subjects: anticarcinogenic activity; Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - isolation & purification; Antineoplastic Agents, Phytogenic - pharmacology; aryltetralin lignan; Berberidaceae; Berberidaceae - chemistry; Berberidaceae - growth & development; Biological and medical sciences; cell growth; chemical structure; Chemistry, Medicinal; cultured cells; Drug Screening Assays, Antitumor; Dysosma versipellis; dysosmarol; General pharmacology; herbaceous plants; Humans; Life Sciences & Biomedicine; lignans; Lignans - chemistry; Lignans - isolation & purification; Lignans - pharmacology; Male; Medical sciences; Molecular Structure; monoepoxylignan; Pharmacognosy. Homeopathy. Health food; Pharmacology & Pharmacy; Pharmacology. Drug treatments; phytochemicals; Plant Roots - chemistry; Plant Sciences; Plants, Medicinal - chemistry; Plants, Medicinal - growth & development; Podophyllotoxin - chemical synthesis; Podophyllum hexandrum; Prostatic Neoplasms - metabolism; Rhizome - chemistry; Science & Technology; spectral analysis; stereochemistry; Tumor Cells, Cultured; China; Asia; ANTITUMOR AGENTS; PODOPHYLLOTOXIN; Cell proliferation; Furan derivatives; HPLC chromatography; Medicinal plant; Sitosterol; Folk medicine; Dicotyledones; Angiospermae; Rhizome; Lignan; Berberidaceae; Tumor cell; Below ground plant part; Human; Root; Kaempferol; Pharmacognosy; In vitro; Biological activity; Podophyllotoxine derivatives; Cell line; Plant origin; Quercetin; Phenols; Isolation; Spermatophyta; Inhibitor; Prostate; Structural analysis
• ISSN: 0163-3864; 1520-6025
• DOI: 10.1021/np060430o

A new monoepoxylignan, dysosmarol (1), along with eight known compounds, podophyllotoxin (2), 4‘-demethylpodophyllotoxin (3), deoxypodophyllotoxin (4), 4‘-demethyldeoxypodophyllotoxin (5), diphyllin (6), kaempferol, quercetin, and β-sitosterol, were isolated from the roots of Dysosma versipellis. The structure of 1 was elucidated by spectroscopic methods. Aryltetralin lignans 2−4 showed the most potent inhibitory activities against the growth of androgen-sensitive (LNCaP) and androgen-independent (PC-3) human prostate cancer cell lines, with IC50 values in the ranges 0.030−0.056 and 0.032−0.082 μM, respectively. A quantitative HPLC analysis showed that compound 2 occurred at the highest concentration in the plant (37.21 mg/g) followed by compound 4 (5.01 mg/g) and compound 3 (2.75 mg/g). Furthermore, D. versipellis roots contain a similar content of compound 2 as compared with the rhizomes and roots of Podophyllum hexandrum, a commercial source of the lignan. Thus, cultivation of D. versipellis in suitable locations may serve as an alternative source for podophyllotoxin (2) production.