Constitutive Activation of MexT by Amino Acid Substitutions Results in MexEF-OprN Overproduction in Clinical Isolates of Pseudomonas aeruginosa

When overproduced, the multidrug efflux system MexEF-OprN increases the resistance of to fluoroquinolones, chloramphenicol, and trimethoprim. In this work, we demonstrate that gain-of-function mutations in the regulatory gene result in oligomerization of the LysR regulator MexT, constitutive upregul...

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Published inAntimicrobial agents and chemotherapy Vol. 62; no. 5
Main Authors Juarez, Paulo, Broutin, Isabelle, Bordi, Christophe, Plésiat, Patrick, Llanes, Catherine
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.05.2018
Subjects
Bacteriology
Life Sciences
Mechanisms of Resistance
Microbiology and Parasitology
Pseudomonas aeruginosa
MexT
Pseudomonas aeruginosa
antibiotic resistance
MexEF-OprN
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Summary:When overproduced, the multidrug efflux system MexEF-OprN increases the resistance of to fluoroquinolones, chloramphenicol, and trimethoprim. In this work, we demonstrate that gain-of-function mutations in the regulatory gene result in oligomerization of the LysR regulator MexT, constitutive upregulation of the efflux pump, and increased resistance in clinical isolates.
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PMCID: PMC5923100
Citation Juarez P, Broutin I, Bordi C, Plésiat P, Llanes C. 2018. Constitutive activation of MexT by amino acid substitutions results in MexEF-OprN overproduction in clinical isolates of Pseudomonas aeruginosa. Antimicrob Agents Chemother 62:e02445-17. https://doi.org/10.1128/AAC.02445-17.
ISSN:0066-4804
1098-6596
DOI:10.1128/aac.02445-17