Constitutive Activation of MexT by Amino Acid Substitutions Results in MexEF-OprN Overproduction in Clinical Isolates of Pseudomonas aeruginosa
When overproduced, the multidrug efflux system MexEF-OprN increases the resistance of to fluoroquinolones, chloramphenicol, and trimethoprim. In this work, we demonstrate that gain-of-function mutations in the regulatory gene result in oligomerization of the LysR regulator MexT, constitutive upregul...
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Published in | Antimicrobial agents and chemotherapy Vol. 62; no. 5 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Society for Microbiology
01.05.2018
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Subjects | |
Online Access | Get full text |
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Summary: | When overproduced, the multidrug efflux system MexEF-OprN increases the resistance of
to fluoroquinolones, chloramphenicol, and trimethoprim. In this work, we demonstrate that gain-of-function mutations in the regulatory gene
result in oligomerization of the LysR regulator MexT, constitutive upregulation of the efflux pump, and increased resistance in clinical isolates. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 PMCID: PMC5923100 Citation Juarez P, Broutin I, Bordi C, Plésiat P, Llanes C. 2018. Constitutive activation of MexT by amino acid substitutions results in MexEF-OprN overproduction in clinical isolates of Pseudomonas aeruginosa. Antimicrob Agents Chemother 62:e02445-17. https://doi.org/10.1128/AAC.02445-17. |
ISSN: | 0066-4804 1098-6596 |
DOI: | 10.1128/aac.02445-17 |