Cryptotomography: reconstructing 3D Fourier intensities from randomly oriented single-shot diffraction patterns

We reconstructed the 3D Fourier intensity distribution of mono-disperse prolate nano-particles using single-shot 2D coherent diffraction patterns collected at DESY's FLASH facility when a bright, coherent, ultrafast X-ray pulse intercepted individual particles of random, unmeasured orientations...

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Bibliographic Details
Published inarXiv.org
Main Authors Loh, N D, Bogan, M, Elser, V, Barty, A, Boutet, S, Bajt, S, Hajdu, J, Ekeberg, T, F R N C Maia, Schulz, J, Seibert, M M, Iwan, B, Timneanu, N, Marchesini, S, Schlichting, I, Shoeman, R L, Lomb, L, Frank, M, Liang, M, Chapman, H N
Format Paper Journal Article
LanguageEnglish
Published Ithaca Cornell University Library, arXiv.org 06.04.2010
Subjects
Biomolecules
Diffraction
Diffraction patterns
Fluence
Physics - Data Analysis, Statistics and Probability
Physics - Optics
Shot
Variations
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Summary:We reconstructed the 3D Fourier intensity distribution of mono-disperse prolate nano-particles using single-shot 2D coherent diffraction patterns collected at DESY's FLASH facility when a bright, coherent, ultrafast X-ray pulse intercepted individual particles of random, unmeasured orientations. This first experimental demonstration of cryptotomography extended the Expansion-Maximization-Compression (EMC) framework to accommodate unmeasured fluctuations in photon fluence and loss of data due to saturation or background scatter. This work is an important step towards realizing single-shot diffraction imaging of single biomolecules.
ISSN:2331-8422
DOI:10.48550/arxiv.1003.0846