Short-fragment Na-DNA dilute aqueous solutions: fundamental length scales and screening

Dielectric spectroscopy is used to investigate fundamental length scales of 146 bp short-fragment (nucleosomal) dilute Na-DNA solutions. Two relaxation modes are detected: the high- and the low-frequency mode. Dependence of the corresponding length scales on the DNA and on the (uni-valent) salt conc...

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Bibliographic Details
Published inarXiv.org
Main Authors Tomic, S, Babic, S Dolanski, Ivek, T, Vuletic, T, Krca, S, Livolant, F, Podgornik, R
Format Paper Journal Article
LanguageEnglish
Published Ithaca Cornell University Library, arXiv.org 31.01.2008
Subjects
Aqueous solutions
Denaturation
Deoxyribonucleic acid
Dependence
Dilution
DNA
Physics - Soft Condensed Matter
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Summary:Dielectric spectroscopy is used to investigate fundamental length scales of 146 bp short-fragment (nucleosomal) dilute Na-DNA solutions. Two relaxation modes are detected: the high- and the low-frequency mode. Dependence of the corresponding length scales on the DNA and on the (uni-valent) salt concentration is studied in detail, being different from the case of long, genomic DNA, investigated before. In low added salt regime, the length scale of the high-frequency mode scales as the average separation between DNAs, though it is smaller in absolute magnitude, whereas the length scale of the low-frequency mode is equal to the contour length of DNA. These fundamental length scales in low added salt regime do not depend on whether DNA is in a double stranded or single stranded form. On the other hand, with increasing added salt, the characteristic length scale of low-frequency mode diminishes at low DNA concentrations probably due to dynamical formation of denaturation bubbles and/or fraying in the vicinity of DNA denaturation threshold.
ISSN:2331-8422
DOI:10.48550/arxiv.0801.4885