Reversible Reaction-Based Fluorescent Probe for Real-Time Imaging of Glutathione Dynamics in Mitochondria

We report a mitochondria-specific glutathione (GSH) probedesignated as Mito-RealThiol (MitoRT)that can monitor in vivo real-time mitochondrial glutathione dynamics, and apply this probe to follow mitochondrial GSH dynamic changes in living cells for the first time. MitoRT can be utilized in confoc...

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Published inACS sensors Vol. 2; no. 9; pp. 1257 - 1261
Main Authors Chen, Jianwei, Jiang, Xiqian, Zhang, Chengwei, MacKenzie, Kevin R, Stossi, Fabio, Palzkill, Timothy, Wang, Meng C, Wang, Jin
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 22.09.2017
Subjects
sensors and probes
flow cytometry
glutathione
mitochondria
fluorescence imaging
oxidative stress
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Summary:We report a mitochondria-specific glutathione (GSH) probedesignated as Mito-RealThiol (MitoRT)that can monitor in vivo real-time mitochondrial glutathione dynamics, and apply this probe to follow mitochondrial GSH dynamic changes in living cells for the first time. MitoRT can be utilized in confocal microscopy, super-resolution fluorescence imaging, and flow cytometry systems. Using MitoRT, we demonstrate that cells have a high priority to maintain the GSH level in mitochondria compared to the cytosol not only under normal growing conditions but also upon oxidative stress.
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Author Contributions: These authors contributed equally.
ISSN:2379-3694
2379-3694
DOI:10.1021/acssensors.7b00425