N‑Terminal Derivatization-Assisted Identification of Individual Amino Acids Using a Biological Nanopore Sensor

Nanopore technology has been employed as a powerful tool for DNA sequencing and analysis. To extend this method to peptide sequencing, a necessary step is to profile individual amino acids (AAs) through their nanopore stochastic signals, which remains a great challenge because of the low signal-to-n...

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Bibliographic Details
Published inACS sensors Vol. 5; no. 6; pp. 1707 - 1716
Main Authors Wei, Xiaojun, Ma, Dumei, Zhang, Zehui, Wang, Leon Y, Gray, Jonathan L, Zhang, Libo, Zhu, Tianyu, Wang, Xiaoqin, Lenhart, Brian J, Yin, Yingwu, Wang, Qian, Liu, Chang
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 26.06.2020
Subjects
Amino Acid Sequence
Amino Acids
Hemolysin Proteins
Nanopores
Peptides
identification
derivatization
proteomics
nanopore
amino acid
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Summary:Nanopore technology has been employed as a powerful tool for DNA sequencing and analysis. To extend this method to peptide sequencing, a necessary step is to profile individual amino acids (AAs) through their nanopore stochastic signals, which remains a great challenge because of the low signal-to-noise ratio and unpredictable conformational changes of AAs during their translocation through nanopores. We showed that the combination of an N-terminal derivatization strategy of AAs with nanopore technology could lead to effective in situ differentiation of AAs. Four different derivatization reactions have been tested with five selected AAs: Ala, Phe, Tyr, His, and Asp. Using an α-hemolysin nanopore, we demonstrated the feasibility of derivatization-assisted identification of AAs regardless of their charge composition and polarity. The method was further applied to discriminate each individual AA in testing data sets using their established nanopore profiles from training data sets. We envision that this proof-of-concept study will not only pave a way for identification of individual AAs but also lead to future applications in protein/peptide sequencing using the nanopore technology.
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Author Contributions
X.W. and D.M. contributed equally to this work.
X.W., D.M., Z.Z., Q.W., and C.L. designed the experiments. Q.W. and C.L. supervised the project: C.L. supervised the nanopore analysis; Q.W. and Y.Y. supervised the AA derivatization and analysis; C.L. and Q.W. supervised the data analysis. X.W. and D.M. primarily performed experiments and data analysis. Z.Z., L.Y.W., J.L.G., L.Z., T.Z., X-Q.W., and B.J.L. helped with experiments and data analysis. X.W., D.M., Y.Y., Q.W. and C.L. wrote the manuscript.
ISSN:2379-3694
2379-3694
DOI:10.1021/acssensors.0c00345