Discovery of Heteroaromatic Sulfones As a New Class of Biologically Compatible Thiol-Selective Reagents
The selective reaction of chemical reagents with reduced protein thiols is critical to biological research. This reaction is utilized to prevent cross-linking of cysteine-containing peptides in common proteomics workflows and is applied widely in discovery and targeted redox investigations of the me...
Saved in:
Published in | ACS chemical biology Vol. 12; no. 8; pp. 2201 - 2208 |
---|---|
Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Chemical Society
18.08.2017
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The selective reaction of chemical reagents with reduced protein thiols is critical to biological research. This reaction is utilized to prevent cross-linking of cysteine-containing peptides in common proteomics workflows and is applied widely in discovery and targeted redox investigations of the mechanisms underlying physiological and pathological processes. However, known and commonly used thiol blocking reagents like iodoacetamide, N-ethylmaleimide, and others were found to cross-react with oxidized protein sulfenic acids (−SOH) introducing significant errors in studies employing these reagents. We have investigated and are reporting here a new heteroaromatic alkylsulfone, 4-(5-methanesulfonyl-[1,2,3,4]tetrazol-1-yl)-phenol (MSTP), as a selective and highly reactive −SH blocking reagent compatible with biological applications. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present Addresses Department of Chemistry, Washington State University Tricities, Richland, Washington 99354 These authors contributed equally. Division of Cardiology, Johns Hopkins School of Medicine, Baltimore, MD 21205 Author Contributions |
ISSN: | 1554-8929 1554-8937 |
DOI: | 10.1021/acschembio.7b00444 |