Association of Immunosuppression and Viral Load With Subcortical Brain Volume in an International Sample of People Living With HIV

• Published in: JAMA network open Vol. 4; no. 1; p. e2031190
• Main Authors: Nir, Talia M, Fouche, Jean-Paul, Ananworanich, Jintanat, Ances, Beau M, Boban, Jasmina, Brew, Bruce J, Chaganti, Joga R, Chang, Linda, Ching, Christopher R K, Cysique, Lucette A, Ernst, Thomas, Faskowitz, Joshua, Gupta, Vikash, Harezlak, Jaroslaw, Heaps-Woodruff, Jodi M, Hinkin, Charles H, Hoare, Jacqueline, Joska, John A, Kallianpur, Kalpana J, Kuhn, Taylor, Lam, Hei Y, Law, Meng, Lebrun-Frénay, Christine, Levine, Andrew J, Mondot, Lydiane, Nakamoto, Beau K, Navia, Bradford A, Pennec, Xavier, Porges, Eric C, Salminen, Lauren E, Shikuma, Cecilia M, Surento, Wesley, Thames, April D, Valcour, Victor, Vassallo, Matteo, Woods, Adam J, Thompson, Paul M, Cohen, Ronald A, Paul, Robert, Stein, Dan J, Jahanshad, Neda
• Format: Journal Article
• Language: English
• Published: United States American Medical Association 15.01.2021
• Subjects: Adult; Aged; Aged, 80 and over; Brain - diagnostic imaging; Brain - pathology; CD4 Lymphocyte Count; Consortia; Cross-Sectional Studies; Female; HIV; HIV Infections - epidemiology; HIV Infections - immunology; HIV Infections - pathology; HIV Infections - virology; Human health and pathology; Human immunodeficiency virus; Humans; Infections; Infectious Diseases; Life Sciences; Magnetic Resonance Imaging; Male; Medical imaging; Middle Aged; Online Only; Original Investigation; Viral Load; Young Adult
• ISSN: 2574-3805; 2574-3805
• DOI: 10.1001/jamanetworkopen.2020.31190

Despite more widely accessible combination antiretroviral therapy (cART), HIV-1 infection remains a global public health challenge. Even in treated patients with chronic HIV infection, neurocognitive impairment often persists, affecting quality of life. Identifying the neuroanatomical pathways associated with infection in vivo may delineate the neuropathologic processes underlying these deficits. However, published neuroimaging findings from relatively small, heterogeneous cohorts are inconsistent, limiting the generalizability of the conclusions drawn to date. To examine structural brain associations with the most commonly collected clinical assessments of HIV burden (CD4+ T-cell count and viral load), which are generalizable across demographically and clinically diverse HIV-infected individuals worldwide. This cross-sectional study established the HIV Working Group within the Enhancing Neuro Imaging Genetics Through Meta Analysis (ENIGMA) consortium to pool and harmonize data from existing HIV neuroimaging studies. In total, data from 1295 HIV-positive adults were contributed from 13 studies across Africa, Asia, Australia, Europe, and North America. Regional and whole brain segmentations were extracted from data sets as contributing studies joined the consortium on a rolling basis from November 1, 2014, to December 31, 2019. Volume estimates for 8 subcortical brain regions were extracted from T1-weighted magnetic resonance images to identify associations with blood plasma markers of current immunosuppression (CD4+ T-cell counts) or detectable plasma viral load (dVL) in HIV-positive participants. Post hoc sensitivity analyses stratified data by cART status. After quality assurance, data from 1203 HIV-positive individuals (mean [SD] age, 45.7 [11.5] years; 880 [73.2%] male; 897 [74.6%] taking cART) remained. Lower current CD4+ cell counts were associated with smaller hippocampal (mean [SE] β = 16.66 [4.72] mm3 per 100 cells/mm3; P < .001) and thalamic (mean [SE] β = 32.24 [8.96] mm3 per 100 cells/mm3; P < .001) volumes and larger ventricles (mean [SE] β = -391.50 [122.58] mm3 per 100 cells/mm3; P = .001); in participants not taking cART, however, lower current CD4+ cell counts were associated with smaller putamen volumes (mean [SE] β = 57.34 [18.78] mm3 per 100 cells/mm3; P = .003). A dVL was associated with smaller hippocampal volumes (d = -0.17; P = .005); in participants taking cART, dVL was also associated with smaller amygdala volumes (d = -0.23; P = .004). In a large-scale international population of HIV-positive individuals, volumes of structures in the limbic system were consistently associated with current plasma markers. Our findings extend beyond the classically implicated regions of the basal ganglia and may represent a generalizable brain signature of HIV infection in the cART era.