Dynamic Cell Fractionation and Transportation Using Moving Dielectrophoresis

• Published in: Analytical chemistry (Washington) Vol. 79; no. 18; pp. 6975 - 6987
• Main Authors: Kua, Chin Hock, Lam, Yee Cheong, Rodriguez, Isabel, Yang, Chun, Youcef-Toumi, Kamal
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 15.09.2007
• Subjects: Analytical chemistry; Cell Fractionation - methods; Cells; Chemistry; Chromatographic methods and physical methods associated with chromatography; Electrocatalysis; Electrodes; Electrophoresis; Exact sciences and technology; Microfluidics - instrumentation; Other chromatographic methods; Saccharomyces cerevisiae; Saccharomyces cerevisiae - chemistry; Saccharomyces cerevisiae - cytology; Studies; Fractionation; Electric field; Sample cell; Dielectrophoresis; Cell; Microfluidics; Operating conditions
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac070810u

This study presents a new cell manipulation method using a moving dielectrophoretic force to transport or fractionate cells along a microfluidic channel. The proposed moving dielectrophoresis (mDEP) is generated by sequentially energizing a single electrode or an array of electrodes to form an electric field that moves cells continuously along the microchannel. Cell fractionation is controlled by the applied electrical frequency, and cell transportation is controlled by the interelectrode activation time. The applicability of this method was demonstrated to simultaneously fractionate and transport Saccharomyces cerevisiae yeast cells, both viable and nonviable, by operating at conditions under which the cells were subjected to positive and negative dielectrophoresis, respectively. Compared to the conventional dielectrophoresis (cDEP and traveling wave dielectrophoresis (twDEP), moving dielectrophoresis allows cells to be separated on the basis of the real part of the Clausius−Mossotti factor, as in cDEP, but yet allows the direct transportation of separated cells without using fluid flow, as in twDEP. This dielectrophoresis technique provides a new way to manipulate cells and can be readily implemented on programmable multielectrode devices.