Development of a droplet digital PCR assay for the detection of BK polyomavirus

• Published in: Microbiology spectrum Vol. 12; no. 11; p. e0108924
• Main Authors: Ai, Lu, Zhao, Yating, Tan, Chianru, Bai, Lu, Huang, Gang, Wang, Ruizhi, Huang, Hao, Yu, Xuegao, Guo, Yong, Chen, Peisong
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 05.11.2024
• Subjects: BK polyomavirus; BK polyomavirus-associated nephropathy; droplet digital PCR; Research Article; Virology; droplet digital PCR; BK polyomavirus-associated nephropathy; BK polyomavirus

It was previously believed that droplet digital polymerase chain reaction had limitations, including high cost, limited throughput, and cumbersome operation, which hindered its widespread application in clinical practice. However, the current fully automated digital PCR platform, combined with strea...