Development of a droplet digital PCR assay for the detection of BK polyomavirus

It was previously believed that droplet digital polymerase chain reaction had limitations, including high cost, limited throughput, and cumbersome operation, which hindered its widespread application in clinical practice. However, the current fully automated digital PCR platform, combined with strea...

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Published inMicrobiology spectrum Vol. 12; no. 11; p. e0108924
Main Authors Ai, Lu, Zhao, Yating, Tan, Chianru, Bai, Lu, Huang, Gang, Wang, Ruizhi, Huang, Hao, Yu, Xuegao, Guo, Yong, Chen, Peisong
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 05.11.2024
Subjects
BK polyomavirus
BK polyomavirus-associated nephropathy
droplet digital PCR
Research Article
Virology
droplet digital PCR
BK polyomavirus-associated nephropathy
BK polyomavirus
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Summary:It was previously believed that droplet digital polymerase chain reaction had limitations, including high cost, limited throughput, and cumbersome operation, which hindered its widespread application in clinical practice. However, the current fully automated digital PCR platform, combined with streamlined operations, can detect 96 samples at once, and the entire process can be completed within an hour, laying a solid foundation for its extensive use.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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Lu Ai, Yating Zhao, and Chianru Tan contributed equally to this article. Author order was determined by drawing straws.
The authors declare no conflict of interest.
ISSN:2165-0497
2165-0497
DOI:10.1128/spectrum.01089-24