Interkingdom Gut Microbiome and Resistome of the Cockroach Blattella germanica

• Published in: mSystems Vol. 6; no. 3
• Main Authors: Domínguez-Santos, Rebeca, Pérez-Cobas, Ana Elena, Cuti, Paolo, Pérez-Brocal, Vicente, García-Ferris, Carlos, Moya, Andrés, Latorre, Amparo, Gil, Rosario
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 11.05.2021
• Subjects: Research Article; gut microbiome; kanamycin; antibiotic resistance genes (ARGs); Blattella germanica; symbiosis
• ISSN: 2379-5077; 2379-5077
• DOI: 10.1128/mSystems.01213-20

For the first time, we analyze the interkingdom hindgut microbiome of this species, including bacteria, fungi, archaea, and viruses. Network analysis reveals putative cooperation between core bacteria that could be key for ecosystem equilibrium. Cockroaches are intriguing animals with two coexisting symbiotic systems, an endosymbiont in the fat body, involved in nitrogen metabolism, and a gut microbiome whose diversity, complexity, role, and developmental dynamics have not been fully elucidated. In this work, we present a metagenomic approach to study Blattella germanica populations not treated, treated with kanamycin, and recovered after treatment, both naturally and by adding feces to the diet, with the aim of better understanding the structure and function of its gut microbiome along the development as well as the characterization of its resistome. IMPORTANCE For the first time, we analyze the interkingdom hindgut microbiome of this species, including bacteria, fungi, archaea, and viruses. Network analysis reveals putative cooperation between core bacteria that could be key for ecosystem equilibrium. We also show how antibiotic treatments alter microbiota diversity and function, while both features are restored after one untreated generation. Combining data from B. germanica treated with three antibiotics, we have characterized this species’ resistome. It includes genes involved in resistance to several broad-spectrum antibiotics frequently used in the clinic. The presence of genetic elements involved in DNA mobilization indicates that they can be transferred among microbiota partners. Therefore, cockroaches can be considered reservoirs of antibiotic resistance genes (ARGs) and potential transmission vectors.