Emergence of Colistin Resistance Gene mcr - 10 in Enterobacterales Isolates Recovered from Fecal Samples of Chickens, Slaughterhouse Workers, and a Nearby Resident

• Published in: Microbiology spectrum Vol. 10; no. 2; p. e0041822
• Main Authors: Xu, Linna, Wan, Fen, Fu, Hao, Tang, Biao, Ruan, Zhi, Xiao, Yonghong, Luo, Qixia
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 27.04.2022
• Subjects: Abattoirs; Animals; Anti-Bacterial Agents - pharmacology; antibiotic resistance; Antimicrobial Chemotherapy; Chickens; Colistin - pharmacology; Drug Resistance, Bacterial - genetics; Enterobacterales; Escherichia coli; Escherichia coli Proteins - genetics; Integrases - genetics; Klebsiella pneumoniae - genetics; mcr-10; Microbial Sensitivity Tests; one health; Plasmids - genetics; polymyxins; Research Article; Tigecycline; Enterobacterales; one health; antibiotic resistance; polymyxins; mcr-10
• ISSN: 2165-0497; 2165-0497
• DOI: 10.1128/spectrum.00418-22

The wide spread of plasmid-borne mobilized colistin resistance ( ) genes from animals to humans broadly challenges the clinical use of polymyxins. Here, we evaluated the incidence of a recently reported variant, - , in animals and humans in the same area. Our results revealed the presence of novel -carrying plasmids in two Klebsiella pneumoniae isolates from chickens, one Escherichia coli isolate from slaughterhouse workers, and a chromosome-borne gene in Enterobacter kobei from a healthy resident in the same region. It is worth mentioning that the multidrug-resistant ST11 K. pneumoniae isolates coharboring - and genes in two separate plasmids not only were resistant to polymyxins (MIC = 8 mg/L) but also showed reduced susceptibility to tigecycline (MIC ≥ 2 mg/L) due to the (A) mutation or the gene cluster. The structure - - -like was found in genetic environments of both the plasmid and chromosome carrying . We compared genomic epidemiological characteristics of -harboring bacteria available in 941,449 genomes in the NCBI database (including strains of K. pneumoniae, E. coli, and ) with isolates in this study. The results indicated a sporadic distribution of all around the world and in a variety of sources, including humans, environments, and animals, which confirms that has spread among various hosts and warrants close monitoring and further future studies. We discovered -harboring isolates in the "one health" approach and reported for the first time multidrug-resistant clinically threatening ST11 K. pneumoniae isolates coharboring - and genes that are resistant to polymyxins and show reduced susceptibility to tigecycline. The exhaustive screening of 941,449 bacterial genomes in the GenBank database discovered a sporadic distribution of -harboring isolates all around the world in a variety of sources, especially humans, which warrants close monitoring and a particular concern in clinical settings.