Antioxidative Activity of Volatile Extracts Isolated from Angelica tenuissimae Roots, Peppermint Leaves, Pine Needles, and Sweet Flag Leaves

• Published in: Journal of agricultural and food chemistry Vol. 53; no. 10; pp. 4124 - 4129
• Main Authors: Ka, Mi-Hyun, Choi, Eun Hye, Chun, Hang-Sook, Lee, Kwang-Geun
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 18.05.2005
• Subjects: Acorus; Acorus - chemistry; Acorus gramineus; Angelica; Angelica - chemistry; Angelica tenuissimae; antioxidant activity; antioxidants; Antioxidants - isolation & purification; Antioxidants - pharmacology; Biological and medical sciences; cell lines; Cell Survival - drug effects; Chromatography, Gas; conifer needles; cytoprotective effect; Gas Chromatography-Mass Spectrometry; General pharmacology; Humans; hydrogen peroxide; Hydrogen Peroxide - pharmacology; leaves; lipid peroxidation; Lipid Peroxidation - drug effects; Medical sciences; medicinal plants; medicinal properties; Mentha arvensis; Mentha piperita - chemistry; Neuroblastoma; neuroblastoma cells; neurons; oxidation; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Pinus sylvestris; Pinus sylvestris - chemistry; plant extracts; Plant Extracts - chemistry; Plant Leaves - chemistry; Plant Roots - chemistry; protective effect; roots; Tumor Cells, Cultured; volatile organic compounds; Volatilization; Angelica tenuissima Nakai; Monocotyledones; Extract; Pinus sylvestris L; Pinus sylvestris; antioxidant activity; Medicinal plant; Plant part; Mentha arvensis; Dicotyledones; Angiospermae; Gymnospermae; Angelica; volatile extracts; Volatile compound; Medicinal plants; Acorus gramineus; Root; Acorus gramineus Rhizoma; Peppermint; Plant leaf; Antioxidant; Mentha arvensis L; Mentha piperita; Araceae; Labiatae; Coniferales; Spermatophyta; Umbelliferae
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf047932x

Volatile extracts were isolated from dried medicinal plants [Angelica tenuissimae roots (AT, Angelica tenuissima Nakai), peppermint leaves (PL, Mentha arvensis L.), pine needles (PN, Pinus sylvestris L.), and sweet flag leaves (SF, Acorus gramineus Rhizoma)] using steam distillation under reduced pressure, followed by continuous liquid−liquid extraction (DRP−LLE). The extracts were then analyzed by gas chromatography (GC) and gas chromatography−mass spectrometry (GC−MS). The major volatile constituents of AT, PL, PN, and SF were 3-butylidene-4,5-dihydrophthalide (32 mg/g), menthol (18 mg/g), thunbergol (2.1 mg/g), and cis-asarone (37 mg/g), respectively. The inhibitory activity (%) of the extracts against hexanal oxidation ranged from 33 to 98% at a level of 50 μg/mL. Among the volatile extracts, PL and PN increased cell viabilities by 10 and 24%, respectively, at a dose of 1 μg/mL, compared to that of H2O2-treated brain neuroblastoma cells, SK-N-SH. However, a 20% reduction in the malonaldehyde level, an index for lipid peroxidation, was observed at only 1 μg/mL concentration of PN. Keywords: Medicinal plants; antioxidant activity; volatile extracts; Angelica tenuissima Nakai; Mentha arvensis L.; Pinus sylvestris L.; Acorus gramineus Rhizoma