Effects of Phosphorus Fertilizer Supplementation on Antioxidant Enzyme Activities in Tomato Fruits

• Published in: Journal of agricultural and food chemistry Vol. 53; no. 5; pp. 1539 - 1545
• Main Authors: Ahn, Taehyun, Oke, Moustapha, Schofield, Andrew, Paliyath, Gopinadhan
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 09.03.2005
• Subjects: Antioxidants; ascorbate peroxidase; Ascorbate Peroxidases; Biological and medical sciences; Feeding. Feeding behavior; fertilizer application; Fertilizers; foliar spraying; Food industries; Fruit - enzymology; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; guaiacol peroxidase; Isoenzymes - metabolism; isozymes; Lycopersicon esculentum - enzymology; Peroxidase - metabolism; peroxidases; Peroxidases - metabolism; Phosphorus - administration & dosage; phosphorus fertilizers; seasonal variation; soil treatment; Solanum lycopersicum var. lycopersicum; superoxide dismutase; Superoxide Dismutase - metabolism; tomatoes; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Vegetables; Ascorbic acid; Fertilization; Fruit; Enzyme; Vitamin; Phosphorus; Superoxide dismutase; Antioxidant; Tomato; Feeding; Fertilizers; Peroxidases; Dicotyledones; Quality; Angiospermae; Lycopersicon esculentum; Spermatophyta; Peroxidase; Oxidoreductases; Solanaceae; Supplementation; Ascorbate peroxidase; fruit quality
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf040248y

The effects of soil and foliar phosphorus supplementation on the activities and levels of superoxide dismutase (SOD), guaiacol peroxidase (POX), and ascorbate peroxidase (APX) in tomato fruits were evaluated by determining enzyme activities and isoenzyme analysis. Both protein levels and enzyme activities varied depending on the variety and season. In general, phosphorus supplementation did not alter SOD, POX, and APX activities significantly;however, some treatments showed season- and stage-specific enhancement in activities as noticed with hydrophos and seniphos supplementation. Three different SOD isozymes were observed, and these isozymes showed very similar staining intensities in response to P application and during the three developmental stages studied. Two major isozymes of POX and two different APX isozymes were observed at all the developmental stages. The results suggest that antioxidant enzyme activities may be influenced by the availability of phosphorus, but are subject to considerable variation depending on the developmental stage and the season. Keywords: Ascorbate peroxidase; fruit quality; Lycopersicon esculentum; peroxidase; superoxide dismutase