A Novel Method of Immobilizing Antibodies on a Quartz Crystal Microbalance Using Plasma-Polymerized Films for Immunosensors

• Published in: Analytical chemistry (Washington) Vol. 68; no. 10; pp. 1695 - 1700
• Main Authors: Nakanishi, Keijiro, Muguruma, Hitoshi, Karube, Isao
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 15.05.1996
• Subjects: Animals; Biological and medical sciences; Biosensing Techniques; Biotechnology; Chemistry; Crystals; Ethylenediamines; Fundamental and applied biological sciences. Psychology; Humans; Immobilization of enzymes and other molecules; Immobilization techniques; Immunoglobulin G; Immunosorbents; Methods. Procedures. Technologies; Polymers; Quartz; Rabbits; Sensors; Serum Albumin - immunology; Performance evaluation; Support; Antibody; Film; Biosensor; Immobilization; Polymerization; Application; Electric discharge; Immunosensor; Quartz microbalance
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac950756u

A novel method of immobilizing antibodies on quartz crystals for use in immunosensors was developed using an ethylenediamine plasma-polymerized film matrix. The films formed on the quartz crystals are extremely thin and homogeneous, and they incorporate amino groups. Sensors produced using this method are more reproducible from sample to sample and exhibit lower noise and higher sensitivity than sensors made using conventional immobilization methods, e.g., via polyethylenimine and (γ-aminopropyl)trimethoxysilane. This is, to our knowledge, the first reported application of plasma-polymerized films to quartz crystal microbalance immunosensors. Results on orientation-controlled immobilization of antibodies, reusability and calibration tests are also presented.