Application of Avidin−Biotin Technology and Adsorptive Transfer Stripping Square-Wave Voltammetry for Detection of DNA Hybridization and Avidin in Transgenic Avidin Maize

• Published in: Analytical chemistry (Washington) Vol. 75; no. 11; pp. 2663 - 2669
• Main Authors: Masarik, Michal, Kizek, Rene, Kramer, Karl J, Billova, Sabina, Brazdova, Marie, Vacek, Jan, Bailey, Michele, Jelen, Frantisek, Howard, John A
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.06.2003
• Subjects: Adsorption; adsorptive transfer stripping square-wave voltammetry; analytical methods; avidin; Avidin - analysis; Avidin - chemistry; Avidin - genetics; bacterial proteins; Biological and medical sciences; biotin; Biotin - chemistry; chickens; corn; Diverse techniques; DNA; DNA - metabolism; Electrochemistry - methods; Electrodes; Fundamental and applied biological sciences. Psychology; Molecular and cellular biology; nucleic acid hybridization; Nucleic Acid Hybridization - methods; oligonucleotides; Plants, Genetically Modified - chemistry; Plants, Genetically Modified - genetics; quantitative analysis; recombinant proteins; streptavidin; Streptavidin - analysis; Streptavidin - chemistry; transgenes; transgenic plants; Zea mays; Zea mays - chemistry; Zea mays - genetics; Adsorption; DNA; Corn; Voltammetry; Biotin; Transgenic plant; Hybridization; Avidin; Square wave
• ISSN: 0003-2700; 1520-6882; 1520-6882
• DOI: 10.1021/ac020788z

The proteins streptavidin and avidin were electrochemically detected in solution by adsorptive transfer stripping square wave voltammetry (AdTS SWV) at a carbon paste electrode (CPE). AdTS SWV was used to quantify biotinylated oligonucleotides, DNA hybridizations, and avidin in extracts of transgenic avidin maize. The detection limits of denatured and native streptavidin were 6 pM and 120 nM, respectively. The results demonstrated that streptavidin/avidin AdTS SWV is a sensitive and specific method for quantifying DNA and proteins in biological samples such as foods and tissue extracts, including genetically modified crops (avidin maize) and other plants in neighboring fields.