Identification of Urinary Metabolites of the Red Clover Isoflavones Formononetin and Biochanin A in Human Subjects

Dietary supplements manufactured from red clover are widely marketed to provide the beneficial health effects of isoflavones without changing the original diet. In this study the metabolism of formononetin and biochanin A, the principal isoflavones of red clover, was studied in human subjects. Seven...

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Published inJournal of agricultural and food chemistry Vol. 52; no. 22; pp. 6802 - 6809
Main Authors Heinonen, Satu-Maarit, Wähälä, Kristiina, Adlercreutz, Herman
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 03.11.2004
Subjects
Adult
biochanin A
Biological and medical sciences
daidzein
Dietary Supplements
Feeding. Feeding behavior
Female
Food industries
formononetin
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry
genistein
Genistein - urine
Humans
ingestion
isoflavones
Isoflavones - urine
metabolism
metabolites
Middle Aged
Research and development. New food products, dietetic foods and beverages
Trifolium - chemistry
Trifolium pratense
urine
Vertebrates: anatomy and physiology, studies on body, several organs or systems
Human
Isoflavone
Urine
Biological fluid
Biochanin A
Nutrition
Metabolite
Genistein
Identification
Trifolium pratense
Metabolism
Flavonoid
Feeding
Phytoestrogen
Polyphenol
Leguminosae
Dicotyledones
Angiospermae
Spermatophyta
Daidzein
Formononetin
Food supplement
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Summary:Dietary supplements manufactured from red clover are widely marketed to provide the beneficial health effects of isoflavones without changing the original diet. In this study the metabolism of formononetin and biochanin A, the principal isoflavones of red clover, was studied in human subjects. Seven women ingested four red clover dietary supplements, and the metabolites of the isoflavones were identified in their urine samples. The structures of trimethylsilyl derivatives of the metabolites were established by GC-MS. New reduced metabolites of formononetin (dihydroformononetin and angolensin) and biochanin A (dihydrobiochanin A and 6‘-hydroxyangolensin) were identified in urine samples using authentic reference compounds. Possible metabolic pathways are presented for the red clover isoflavones formononetin and biochanin A. Keywords: Biochanin A; formononetin; daidzein; genistein; metabolism
Bibliography:istex:7049C4970DB3B799732FF080746BBDE9F1F95095
ark:/67375/TPS-42GJM9C3-Z
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0021-8561
1520-5118
DOI:10.1021/jf0492767