A Modular Vector Toolkit with a Tailored Set of Thermosensors To Regulate Gene Expression in Thermus thermophilus

Modular plasmid architectures have shown to be a very useful resource to standardize, build, share, and compare biological parts and functional vectors, and are being applied in an increasing number of microorganisms. Here, we present a modular plasmid toolkit for Thermus thermophilus, a species con...

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Published inACS omega Vol. 4; no. 11; pp. 14626 - 14632
Main Authors Verdú, Carlos, Sanchez, Esther, Ortega, Carmen, Hidalgo, Aurelio, Berenguer, José, Mencía, Mario
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 10.09.2019
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Summary:Modular plasmid architectures have shown to be a very useful resource to standardize, build, share, and compare biological parts and functional vectors, and are being applied in an increasing number of microorganisms. Here, we present a modular plasmid toolkit for Thermus thermophilus, a species considered as a workhorse for biotechnology and a model for high-temperature biology. Apart from integrating improved versions of already existing parts, we have characterized specific promoters and developed a thermosensor-based palette that restricts the expression to Thermus and, at the same time, controls protein expression in this organism in a temperature-dependent manner.
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ISSN:2470-1343
2470-1343
DOI:10.1021/acsomega.9b02107