Multiplexed and Reiterative Fluorescence Labeling via DNA Circuitry
A class of reactive DNA circuits was adapted as erasable molecular imaging probes that allow fluorescent reporting complexes to be assembled and disassembled on a biological specimen. Circuit reactions are sequence-dependent and therefore facilitate multiplexed (multicolor) detection. Yet, the abili...
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Published in | Bioconjugate chemistry Vol. 21; no. 12; pp. 2327 - 2331 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Chemical Society
15.12.2010
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Subjects | |
Online Access | Get full text |
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Summary: | A class of reactive DNA circuits was adapted as erasable molecular imaging probes that allow fluorescent reporting complexes to be assembled and disassembled on a biological specimen. Circuit reactions are sequence-dependent and therefore facilitate multiplexed (multicolor) detection. Yet, the ability to disassemble reporting complexes also allows fluorophores to be removed and new circuit complexes to be used to label additional markers. Thus, these probes present opportunities to increase the total number of molecular targets that can be visualized on a biological sample by allowing multiple rounds of fluorescence microscopy to be performed. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Department of Chemistry, Rice University. Department of Bioengineering, Rice University. M. D. Anderson Cancer Center. |
ISSN: | 1043-1802 1520-4812 |
DOI: | 10.1021/bc100348q |