Multiplexed and Reiterative Fluorescence Labeling via DNA Circuitry

A class of reactive DNA circuits was adapted as erasable molecular imaging probes that allow fluorescent reporting complexes to be assembled and disassembled on a biological specimen. Circuit reactions are sequence-dependent and therefore facilitate multiplexed (multicolor) detection. Yet, the abili...

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Bibliographic Details
Published inBioconjugate chemistry Vol. 21; no. 12; pp. 2327 - 2331
Main Authors Duose, Dzifa Y., Schweller, Ryan M., Hittelman, Walter N., Diehl, Michael R.
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 15.12.2010
Subjects
Base Pairing
Biomarkers
Biomarkers - analysis
Deoxyribonucleic acid
DNA
DNA - metabolism
Electrophoresis, Polyacrylamide Gel
Entropy
Female
Fluorescence
Fluorescent Dyes - chemistry
Fluorescent Dyes - metabolism
HeLa Cells
Humans
Microscopy
Microscopy, Fluorescence
Molecular Imaging - methods
Molecular Probes - chemistry
Molecular Probes - metabolism
Oligonucleotide Array Sequence Analysis - methods
Staining and Labeling - methods
Thermodynamics
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Summary:A class of reactive DNA circuits was adapted as erasable molecular imaging probes that allow fluorescent reporting complexes to be assembled and disassembled on a biological specimen. Circuit reactions are sequence-dependent and therefore facilitate multiplexed (multicolor) detection. Yet, the ability to disassemble reporting complexes also allows fluorophores to be removed and new circuit complexes to be used to label additional markers. Thus, these probes present opportunities to increase the total number of molecular targets that can be visualized on a biological sample by allowing multiple rounds of fluorescence microscopy to be performed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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Department of Chemistry, Rice University.
Department of Bioengineering, Rice University.
M. D. Anderson Cancer Center.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc100348q