Genomic insights into virulence, antimicrobial resistance, and adaptation acumen of Escherichia coli isolated from an urban environment

Evolutionary patterns of E. coli bacteria convey that they evolve into highly pathogenic forms by acquiring fitness advantages, such as AMR, and various virulence factors through the horizontal gene transfer (HGT)-mediated acquisition of MGEs. However, limited research on the genetic profiles of env...

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Published inmBio Vol. 15; no. 3; p. e0354523
Main Authors Saini, Poorvi, Bandsode, Viraj, Singh, Anuradha, Mendem, Suresh Kumar, Semmler, Torsten, Alam, Munirul, Ahmed, Niyaz
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 13.03.2024
Subjects
Animals
Anti-Bacterial Agents - pharmacology
antimicrobial resistance
Drug Resistance, Bacterial
environment
Environmental Microbiology
Escherichia coli
Escherichia coli - genetics
Escherichia coli Infections - microbiology
genome analysis
Genomics
Humans
Research Article
Soil
virulence
Virulence - genetics
Water
environment
virulence
antimicrobial resistance
Escherichia coli
genome analysis
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Summary:Evolutionary patterns of E. coli bacteria convey that they evolve into highly pathogenic forms by acquiring fitness advantages, such as AMR, and various virulence factors through the horizontal gene transfer (HGT)-mediated acquisition of MGEs. However, limited research on the genetic profiles of environmental E. coli , particularly from India, hinders our understanding of their transition to pathogenic forms and impedes the adoption of a comprehensive approach to address the connection between environmentally dwelling E. coli populations and human and veterinary public health. This study focuses on high-resolution genomic analysis of the environmental E. coli isolates aiming to understand the genetic similarities and differences among isolates from different environmental niches and uncover the survival strategies employed by these bacteria to thrive in their surroundings. Our approach involved molecular characterization of environmental samples using PCR-based DNA fingerprinting and subsequent WGS analysis. This multidisciplinary approach is likely to provide valuable insights into the understanding of any potential spill-over to human and animal populations and locales. Investigating these environmental isolates has significant potential for developing epidemiological strategies against transmission and understanding niche-specific evolutionary patterns.
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The authors declare no conflict of interest.
Poorvi Saini and Viraj Bandsode contributed equally to this article. The order of names was decided based on mutual agreement.
ISSN:2150-7511
2150-7511
DOI:10.1128/mbio.03545-23