Integrated metagenomic and metaproteomic analyses reveal bacterial micro-ecological mechanisms in coral bleaching

• Published in: mSystems Vol. 8; no. 6; p. e0050523
• Main Authors: Cheng, Keke, Li, Xinyang, Tong, Mengmeng, Jong, Mui-Choo, Cai, Zhonghua, Zheng, Huina, Xiao, Baohua, Zhou, Jin
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 21.12.2023
• Subjects: Algae; Animals; Anthozoa - metabolism; Apoptosis; Bacteria; Bacteria - genetics; Biosynthesis; Carbohydrate metabolism; Cell cycle; Chlorophyll; Coral Bleaching; Coral Reefs; coral symbionts; coral-associated bacteria; Corals; Energy Metabolism; Environmental Microbiology; Fungi; Gluconeogenesis; Heat; Heat stress; High temperature; Information storage; Metabolism; Metabolites; metagenome; Metagenomics; metaproteome; Molecular modelling; Opportunist infection; Pathogens; Photosynthesis; Physiology; Probiotics; Research Article; Signal transduction; Stress response; Symbionts; Temperature effects; Virulence; Virulence factors; Hainan Island; metagenome; coral symbionts; metaproteome; coral bleaching; coral-associated bacteria
• ISSN: 2379-5077; 2379-5077
• DOI: 10.1128/msystems.00505-23

Coral reefs worldwide are facing rapid decline due to coral bleaching. However, knowledge of the physiological characteristics and molecular mechanisms of coral symbionts respond to stress is scarce. Here, metagenomic and metaproteomic approaches were utilized to shed light on the changes in the composition and functions of coral symbiotic bacteria during coral bleaching. The results demonstrated that coral bleaching significantly affected the composition of symbionts, with bacterial communities dominating in bleached corals. Through differential analyses of gene and protein expression, it becomes evident that symbionts experience functional disturbances in response to heat stress. These disturbances result in abnormal energy metabolism, which could potentially compromise the health and resilience of the symbionts. Furthermore, our findings highlighted the highly diverse microbial communities of coral symbionts, with beneficial bacteria providing critical services to corals in stress responses and pathogenic bacteria driving coral bleaching. This study provides comprehensive insights into the complex response mechanisms of coral symbionts under heat stress from the micro-ecological perspective and offers fundamental data for future monitoring of coral health.