Quantum Dot-Based Western Blot Technology for Ultrasensitive Detection of Tracer Proteins

The present study describes two quantum dot-based Western blotting protocols for ultrasensitive detection of “tracer” proteins in cell lysates.

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 127; no. 26; pp. 9328 - 9329
Main Authors Bakalova, Rumiana, Zhelev, Zhivko, Ohba, Hideki, Baba, Yoshinobu
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 06.07.2005
Subjects
Actins - analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Blotting, Western - methods
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Humans
Nanotechnology - methods
Proteins
Proteins - analysis
Quantum Dots
Sensitivity and Specificity
Telomere-Binding Proteins - analysis
Telomeric Repeat Binding Protein 1 - analysis
Immunoprecipitation reaction
Semiconductor quantum dots
Biochemical analysis
Fluorescent tracer
Sample preparation
II-VI semiconductors
Immunoblotting assay
Biological macromolecule
Quantitative analysis
Cadmium selenides
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Summary:The present study describes two quantum dot-based Western blotting protocols for ultrasensitive detection of “tracer” proteins in cell lysates.
Bibliography:istex:A24AC451AB055DF282C93681B015580432185F84
ark:/67375/TPS-7K4G209M-C
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0002-7863
1520-5126
DOI:10.1021/ja0510055