Self-Assembly/Aggregation Behavior and Adsorption of Enamel Matrix Derivate Protein to Silica Surfaces

• Published in: Langmuir Vol. 22; no. 5; pp. 2227 - 2234
• Main Authors: Halthur, Tobias J, Björklund, Anna, Elofsson, Ulla M
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 28.02.2006
• Subjects: Adsorption; Amelogenin - chemistry; Animals; Chemistry; Coated Materials, Biocompatible; Crystals; Dental Enamel Proteins - chemistry; Dynamic light scattering (DLS); Enamel matrix derivate (EMD); Exact sciences and technology; Fysikalisk kemi; Gel-like films; General and physical chemistry; Hydrogen-Ion Concentration; In Vitro Techniques; Kemi; Light scattering; Multilayer/aggregate formation; Multiprotein Complexes; NATURAL SCIENCES; NATURVETENSKAP; Physical chemistry; Proteins; Quartz; Silica; Silicon Dioxide; Surface and colloid chemistry; Surface physical chemistry; Surface Plasmon Resonance; Surface Properties; Swine; Temperature; Yt- och kolloidkemi; Self assembly; Multilayer; Film; Light scattering; In situ; Crystals; Silica; Protein; Ellipsometry; Aggregation; Particle; Substrate; Adsorption; Sequential; Dynamics; pH; Nanosphere; Aggregate; Quartz microbalance
• ISSN: 0743-7463; 1520-5827; 1520-5827
• DOI: 10.1021/la0525123

Adsorption of the amelogein protein mixture enamel matrix derivate (EMD) to silica surfaces has been studied by in situ ellipsometry and quartz crystal microbalance with dissipation (QCM-D). The protein was found to adsorb as nanospheres in mono- or multilayers, depending on the concentration of “free” nanospheres available in solution. The concentration of free nanospheres is determined by the competitive processes of adsorption and rapid aggregation into microscopic particles, measured by dynamic light scattering (DLS). Multilayers could also be formed by sequential injections of fresh EMD solution. At higher temperature, an up to 6 times thicker gel-like film was formed on the substrate surface, and decreasing the pH lead to disruption of the multilayer/aggregate formation and a decreased amount adsorbed.