Nanometric Protein Arrays on Protein-Resistant Monolayers on Silicon Surfaces

• Published in: Journal of the American Chemical Society Vol. 126; no. 26; pp. 8098 - 8099
• Main Authors: Gu, Jianhua, Yam, Chi Ming, Li, Sha, Cai, Chengzhi
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 07.07.2004; Amer Chemical Soc
• Subjects: Avidin - chemistry; Biological and medical sciences; Chemistry; Chemistry, Multidisciplinary; Fundamental and applied biological sciences. Psychology; Molecular biophysics; Nanotechnology; Physical Sciences; Polyethylene Glycols - chemistry; Protein Array Analysis; Science & Technology; Silicon Dioxide - chemistry; Surface Properties; Surface properties. Adsorption; SELF-ASSEMBLED MONOLAYERS; DIP-PEN NANOLITHOGRAPHY; CHEMISTRY; NANOSTRUCTURES; FABRICATION; IMMOBILIZATION; LITHOGRAPHY; TEMPLATES; Ethylene oxide polymer; Monolayer; Atomic force microscopy; Liquid solid interface; Liquid solid adsorption; Organic adsorbate; Adsorption capacity; Friction coefficient; Crystal face; Biological macromolecule; Silicon; Inorganic adsorbent
• ISSN: 0002-7863; 1520-5126
• DOI: 10.1021/ja048405x

We present a novel approach for preparation of nanometric protein arrays, based on binding of avidin molecules to nanotemplates generated by conductive AFM lithography on robust oligo(ethylene glycol)-terminated monolayers on silicon (111) surfaces that are protein-resistant. We showed that only biotinated-BSA but not the native BSA bind to the avidin arrays and that the resulting arrays of biotinated BSA could bind avidin to form protein dots with a feature size of ∼30 nm. This result demonstrates that the avidin array may serve as templates for preparation of nanoarrays of a wide variety of biotin-tagged proteins for studying their interactions with other protein molecules at nanoscale.