Novel phage infecting the Roseobacter CHUG lineage reveals a diverse and globally distributed phage family

• Published in: mSphere Vol. 9; no. 7; p. e0045824
• Main Authors: Wu, Zuqing, Guo, Luyuan, Wu, Ying, Yang, Mingyu, Du, Sen, Shao, Jiabing, Zhang, Zefeng, Zhao, Yanlin
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 30.07.2024
• Subjects: Activated carbon; Amino acids; Bacteriophages; Bacteriophages - classification; Bacteriophages - genetics; Bacteriophages - isolation & purification; Biogeography; Carbon cycle; CHUG lineage; CHUG phages; ecological distribution; Evolutionary conservation; Gene mapping; Genes; Genetic diversity; genome sequence; Genome, Viral; Genomes; Genomic analysis; Genomics; Integrase; Marine environment; Metabolism; Oceans; Phages; Phylogenetics; Phylogeny; Prophages; Prophages - classification; Prophages - genetics; Prophages - isolation & purification; Proteins; Research Article; Roseobacter; Roseobacter - classification; Roseobacter - genetics; Roseobacter - virology; Seawater - microbiology; Seawater - virology; Synteny; CHUG phages; genome sequence; ecological distribution; CHUG lineage; Roseobacter
• ISSN: 2379-5042; 2379-5042
• DOI: 10.1128/msphere.00458-24

The Roseobacter CHUG lineage, affiliated with the Pelagic Roseobacter Cluster (PRC), is widely distributed in the global oceans and is active in oligotrophic seawater. However, knowledge of the bacteriophages that infect CHUG members is limited. In this study, a CHUG phage, CRP-810, that infects the CHUG strain FZCC0198, was isolated and shown to have a novel genomic architecture. In addition, 251 uncultured viral genomes closely related to CRP-810 were recovered and included in the analyses. Phylogenomic analyses revealed that the CRP-810-type phages represent a new phage family containing at least eight genus-level subgroups. Members of this family were predicted to infect various marine bacteria. We also demonstrated that the CRP-810-type phages are widely distributed in global oceans and display distinct biogeographic patterns related to latitude. Collectively, this study provides important insights into the genomic organization, diversity, and ecology of a novel phage family that infect ecologically important bacteria in the global ocean.