Ni/NiO Core/Shell Nanoparticles for Selective Binding and Magnetic Separation of Histidine-Tagged Proteins

Ni/NiO core/shell nanoparticles having high affinity with polyhistidine were synthesized by decomposition of a Ni surfactant complex followed by air oxidation. Ni/NiO nanoparticles showed selective and efficient binding to histidine-tagged proteins and easy separation by using a magnet. These provid...

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 128; no. 33; pp. 10658 - 10659
Main Authors Lee, In Su, Lee, Nohyun, Park, Jongnam, Kim, Byung Hyo, Yi, Yong-Weon, Kim, Taeuk, Kim, Tae Kook, Lee, In Hwan, Paik, Seung R, Hyeon, Taeghwan
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 23.08.2006
Subjects
Histidine - chemistry
Magnetics
Metal Nanoparticles - chemistry
Nickel - chemistry
Protein Binding
Proteins - chemistry
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Summary:Ni/NiO core/shell nanoparticles having high affinity with polyhistidine were synthesized by decomposition of a Ni surfactant complex followed by air oxidation. Ni/NiO nanoparticles showed selective and efficient binding to histidine-tagged proteins and easy separation by using a magnet. These provided a more convenient way to efficient purification of histidine-tagged proteins compared with the conventional Ni−NTA complex-bound resins and microbeads.
Bibliography:ark:/67375/TPS-WPVGS28B-X
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ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0002-7863
1520-5126
DOI:10.1021/ja063177n