Changing Selectivity of DNA Oxidation from Deoxyribose to Guanine by Ligand Design and a New Binuclear Copper Complex
A dinuclear copper complex [CuII 2(PD‘O)(H2O)2]3+ (1) (where PD‘OH is a pyridylalkylamine containing binucleating ligand) promotes guanine oxidation in single-stranded DNA in the presence of 3-mercaptopropionic acid and dioxygen. This reaction is detected after subsequent piperidine treatment. Littl...
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Published in | Journal of the American Chemical Society Vol. 127; no. 2; pp. 520 - 521 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
American Chemical Society
19.01.2005
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Subjects | |
Online Access | Get full text |
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Summary: | A dinuclear copper complex [CuII 2(PD‘O)(H2O)2]3+ (1) (where PD‘OH is a pyridylalkylamine containing binucleating ligand) promotes guanine oxidation in single-stranded DNA in the presence of 3-mercaptopropionic acid and dioxygen. This reaction is detected after subsequent piperidine treatment. Little spontaneous strand scission indicative of deoxyribose oxidation is observed in contrast to the results known for other copper complexes. Chemical characterization and nanospray ionization mass spectrometry analysis of oligodeoxynucleotides treated with 1 suggest conversion of guanine residues to their 2,6-diamino-5-formamidino-4-hydroxypyrimidine (+18 amu) and possibly 5,8-dihydroxy-7,8-dihydroguanine (+34 amu) derivatives. The selectivity toward nucleobase rather than deoxyribose oxidation is discussed in terms of the specific nature of the dicopper (hydro)peroxo species formed with the PD‘OH ligand versus the intermediates formed in the presence of other binucleating ligands. |
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Bibliography: | ark:/67375/TPS-P8S529D6-2 istex:C3783A0A15474BF8A6F704F41694E0A19E360306 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0002-7863 1520-5126 |
DOI: | 10.1021/ja044209e |