Label-free Capacitive Diagnostics: Exploiting Local Redox Probe State Occupancy

An electrode surface confined redox group contributes to a substantial potential-dependent interfacial charging that can be sensitively probed and frequency-resolved by impedance-derived capacitance spectroscopy. In utilizing the sensitivity of this charging fingerprint to redox group environment, o...

Full description

Saved in:
Bibliographic Details
Published inAnalytical chemistry (Washington) Vol. 86; no. 5; pp. 2559 - 2564
Main Authors Lehr, Joshua, Fernandes, Flávio C. Bedatty, Bueno, Paulo R., Davis, Jason J.
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 04.03.2014
Subjects
Acid phosphatase
Antibodies
Charging
Diagnostic systems
Diagnostics
Electrodes
Human
Humans
Markers
Molecular Probes
Oxidation-Reduction
Proteins
Receptors
Sensitivity
Spectrum analysis
Online AccessGet full text

Cover

More Information
Summary:An electrode surface confined redox group contributes to a substantial potential-dependent interfacial charging that can be sensitively probed and frequency-resolved by impedance-derived capacitance spectroscopy. In utilizing the sensitivity of this charging fingerprint to redox group environment, one can seek to generate derived sensory configurations. Exemplified here through the generation of mixed molecular films comprising ferrocene and antibody receptors to two clinically important targets, the label-free methodology is able to report on human prostatic acid phosphatase (PAP), a tumor marker, with a limit of detection of 11 pM and C-reactive protein with a limit of detection of 28 pM. Both assays exhibit linear ranges encompassing those of clinical value.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-2700
1520-6882
DOI:10.1021/ac403727h