Purification and Characterization of a Corrinoid Compound from Chlorella Tablets as an Algal Health Food

Vitamin B12 content of an algal health food, Chlorella tablets (Chlorella sp.), was determined by both Lactobacillus leichmannii ATCC 7830 microbiological and intrinsic factor-chemiluminescence methods. The values of 200.9−211.6 μg/100 g dry weight determined by the chemiluminescence method were sim...

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Bibliographic Details
Published inJournal of agricultural and food chemistry Vol. 50; no. 17; pp. 4994 - 4997
Main Authors Kittaka-Katsura, Hiromi, Fujita, Tomoyuki, Watanabe, Fumio, Nakano, Yoshihisa
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 14.08.2002
Subjects
Biological and medical sciences
Chlorella
Chlorella - chemistry
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Corrinoids
Food, Organic - analysis
General pharmacology
health food
Lactobacillus leichmannii
Luminescent Measurements
Magnetic Resonance Spectroscopy
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Porphyrins - chemistry
Porphyrins - isolation & purification
Spectrophotometry, Ultraviolet
Vitamin B 12 - chemistry
Vitamin B 12 - isolation & purification
vitamin B12
Chlorella
Purification
Pharmacognosy
Algae
Chemiluminescence
Bioavailability
Chlorophyceae
Cyanocobalamin
Characterization
Chlorophyta
Health food
Bacteria
Lactobacillaceae
Tablet
Lactobacillus delbrueckii subsp. lactis
Measurement method
Thallophyta
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Summary:Vitamin B12 content of an algal health food, Chlorella tablets (Chlorella sp.), was determined by both Lactobacillus leichmannii ATCC 7830 microbiological and intrinsic factor-chemiluminescence methods. The values of 200.9−211.6 μg/100 g dry weight determined by the chemiluminescence method were similar to the values (201.3−285.7 μg/100 g dry weight) determined by the microbiological method. A corrinoid compound was purified to homogeneity from the Chlorella tablets and characterized. The purified corrinoid compound was identified as vitamin B12, on the basis of silica gel 60 TLC, C18 reversed-phase HPLC, 1H NMR spectroscopy, and UV−Vis spectroscopy. Keywords: Vitamin B12; algal health food; Chlorella tablets; intrinsic factor; Lactobacillus leichmannii
Bibliography:ark:/67375/TPS-3N5XZJVK-Q
istex:636B1E381965E16172275F376E3E605A6EF30E3A
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0021-8561
1520-5118
DOI:10.1021/jf020345w