Biomimetic Doxorubicin Loaded Polymersomes from Hyaluronan-block-Poly(γ-benzyl glutamate) Copolymers

• Published in: Biomacromolecules Vol. 10; no. 10; pp. 2802 - 2808
• Main Authors: Upadhyay, K. Kumar, Meins, J.-F. Le, Misra, A, Voisin, P, Bouchaud, V, Ibarboure, E, Schatz, C, Lecommandoux, S
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 12.10.2009
• Subjects: Aminoacid polymers; Antineoplastic Agents - chemistry; Applied sciences; Biological and medical sciences; Brain Neoplasms - pathology; Cell Line, Tumor; Chemical Sciences; Doxorubicin - chemistry; Exact sciences and technology; General pharmacology; Glioma - pathology; Humans; Hyaluronic Acid - chemistry; Medical sciences; Molecular Mimicry; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Physicochemistry of polymers; Polyglutamic Acid - analogs & derivatives; Polyglutamic Acid - chemistry; Polymers; Polymers - chemistry; Synthetic biopolymers; Antineoplastic agent; 1,3-Dipolar cycloaddition; Aminoacid copolymer; Cytotoxicity; Drug carrier; Gamma benzyl glutamate copolymer; Doxorubicin; Gamma benzyl glutamate polymer; Mechanism; Hyaluronic acid derivative; Tumor cell; Release; Amphiphilic polymer; Vesicle; Chemical precipitation; Control release polymer; Experimental study; In vitro; Chemical coupling; Glioma; Internalization; Preparation; Diblock copolymer; Oside polymer; Kinetics; Hyaluronic acid; block copolymer; vesicle; polypeptide; targeting; polysaccharide; biomimetic
• ISSN: 1525-7797; 1526-4602
• DOI: 10.1021/bm9006419

Using “click chemistry” as an easy and versatile synthetic strategy to combine hyaluronan and polyglutamate blocks, we have prepared nanovesicles (polymersomes) that present a controlled size, excellent colloidal stability, and a high loading capacity for hydrophilic and hydrophobic drugs. The unique feature of our concept is the use of hyaluronan, a polysaccharide with known capacity for targeting cancer-related protein receptors, as the hydrophilic portion of a block copolymer system. The cytotoxicity and internalization mechanism of doxorubicin-loaded polymersomes have been evaluated in C6 glioma tumor cell lines. The dual purpose served by hyaluronan, as both a hydrophilic block critical to vesicle formation and a binding agent for biological targets, breaks new ground in terms of multifunctional nanomaterial design for drug delivery.