Endothelin-1 stimulates human monocytes in vitro to release TNF-α, IL-1β and IL-6

• Published in: Mediators of Inflammation Vol. 1993; no. 6; pp. 417 - 422
• Main Authors: Helset, E., Sildnes, T., Seljelid, R., Konopski, Z.S.
• Format: Journal Article
• Language: English
• Published: Hindawi Limiteds 1993; Hindawi Publishing Corporation; Hindawi Limited
• Subjects: Monocytes; Cytokines; TNF-α; Endothelin-1; Inflammation; IL-1β; Macrophages; IL-6
• ISSN: 0962-9351; 1466-1861
• DOI: 10.1155/S0962935193000596

INCREASED plasma- and tissue levels of endothelin-1 (ET- 1) during inflammatory diseases, have suggested a role of ET-1 in the pathophysiology of inflammatory reactions. The authors have studied the effect of ET-1 on cytokine release from monocytes and monocyte-derived macrophages. ET-1 increased secretion of TNF-α, IL-1β and IL-6 in a dose- and time-dependent manner. Optimal ET-1 concentration ranged from 0.01 to 1 nM. The maximal response was a 200 to 400% increase in cytokine release. A time-course study revealed that the pattern of cytokines induced by ET-1 was different in monocytes and macrophages, although an early increase in TNF-α was observed in both monocyte and macrophage supernatants. In conclusion, ET-1 stimulates monocytes and macrophages to release cytokines thereby demonstrating a potential role for ET-1 in regulation of inflammatory responses.