Chemical composition, acute toxicity, antioxidant and anti-inflammatory activities of Moroccan Tetraclinis articulata L

• Published in: Journal of Traditional and Complementary Medicine Vol. 7; no. 3; pp. 281 - 287
• Main Authors: El Jemli, Meryem, Kamal, Rabie, Marmouzi, Ilias, Doukkali, Zouhra, Bouidida, El Houcine, Touati, Driss, Nejjari, Rachid, El Guessabi, Lahcen, Cherrah, Yahia, Alaoui, Katim
• Format: Journal Article
• Language: English
• Published: Netherlands 國立臺灣大學食品與生物分子研究中心 01.07.2017; Elsevier Taiwan LLC; Elsevier
• Subjects: Anti-inflammatory activity; Antioxidant potential; Essential oil; GC–MS analysis; Original; T. articulata; Toxicity; Anti-inflammatory activity; Essential oil; GC-MS analysis; Toxicity; T. articulata; Antioxidant potential; DPPH; EO; BHT; T. articulata; LD50; SD; TEAC; IC50; FRAP; GC/MS; GC–MS analysis; DPPH, 2,2-diphenyl-1-picrylhydrazyl radical-scavenging assay; GC/MS, Gas chromatography/mass spectrometry; LD50, lethal dose of 50; FRAP, Ferric-reducing antioxidant power; BHT, Butylatedhydroxytoluene; EO, Essential oil; IC50, Concentration providing 50% inhibition; TEAC, Trolox equivalent antioxidant capacity; SD, Standard deviation; T. articulata, Tetraclinis articulata
• ISSN: 2225-4110; 2225-4110
• DOI: 10.1016/j.jtcme.2016.06.006

Hydro-distilled essential oil (EO) from the leaves of the western Mediterranean and Moroccan endemic plant Tetraclinis articulata was analyzed by GC/MS and examined for its acute toxicity on mice, in order to establish the safe doses. Furthermore, the anti-Inflammatory activity was evaluated based on carrageenan and trauma induced rats paw edema and the antioxidant potential has been investigated using different methods including DPPH radical-scavenging assay, Trolox equivalent antioxidant capacity (TEAC) and Ferric-reducing antioxidant power assay (FRAP). The major identified compounds in GC/MS analysis were bornyl acetate (26.81%), camphor (22.40%) and α-pinene (7.16%), with 25 other minor constituents. No mortalities in acute toxicity were observed, indicating that the LD_(50) of T. articulata essential oil is highest than 5 g/kg. In the anti-inflammatory test based on chemical and mechanical induced trauma, the EO demonstrated an effective reduce swelling by 64.71 ± 9.38% and 69.09 ± 6.02% respectively obtained 6 h after administration at the dose of 200 mg/kg when compared to the control groups. Moreover in the antioxidant testing battery, T. articulata essential oil showed a promising scavenging effect measured by DPPH, TEAC and ferric-reducing power assays with IC_(50) values of 12.05 ± 0.24 mg/mL, 8.90 ± 0.17 mg/mL and 0.15 ± 0.01 mg/mL respectively. These results suggest that, the EO from the leaves of T. articulata constitutes a valuable source of anti-inflammatory and antioxidant metabolites. These findings argue for the possible integration of this oil in pharmaceutical, cosmetic and food industries.