Fiber-Optic SPR Immunosensors Tailored To Target Epithelial Cells through Membrane Receptors

• Published in: Analytical chemistry (Washington) Vol. 87; no. 12; pp. 5957 - 5965
• Main Authors: Malachovská, Viera, Ribaut, Clotilde, Voisin, Valérie, Surin, Mathieu, Leclère, Philippe, Wattiez, Ruddy, Caucheteur, Christophe
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 16.06.2015
• Subjects: Analytical chemistry; Biosensing Techniques; Cells, Cultured; Cellular; Diagnosis; Epidermal growth factor; Epithelial Cells - chemistry; Fiber Optic Technology; Fiber optics; Fibers; Humans; Immunoassay; Immunosensors; Membranes; Microscopy, Atomic Force; Optical fibers; Proteins; Real time; Receptor, Epidermal Growth Factor - analysis; Receptors; Sensors; Surface Plasmon Resonance
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/acs.analchem.5b00159

We report, for the first time, the use of a surface plasmon resonance (SPR) fiber-optic immunosensor for selective cellular detection through membrane protein targeting. The sensor architecture lies on gold-coated tilted fiber Bragg gratings (Au-coated TFBGs) photoimprinted in the fiber core via a laser technique. TFBGs operate in the near-infrared wavelength range at ∼1550 nm, yielding optical and SPR sensing characteristics that are advantageous for the analyses of cellular bindings and technical compatibility with relatively low-cost telecommunication-grade measurement devices. In this work, we take consider their numerous assets to figure out their ability to selectively detect intact epithelial cells as analytes in cell suspensions in the range of 2–5 × 106 cells mL–1. For this, the probe was first thermally annealed to ensure a strong adhesion of the metallic coating to the fiber surface. Its surface was then functionalized with specific monoclonal antibodies via alkanethiol self-assembled monolayers (SAMs) against extracellular domain of epidermal growth factor receptors (EGFRs) and characterized by peak force tapping atomic force microscopy. A differential diagnosis has been demonstrated between two model systems. The developed immunosensors were able to monitor, in real time, the specific attachment of single intact cells in concentrations from 3 × 106 cells mL–1. Such results confirm that the developed probe fits the lab-on-fiber technology and has the potential to be used as a disposable device for in situ and real-time clinical diagnosis.