Exploiting the Twisted Intramolecular Charge Transfer Effect to Construct a Wash-Free Solvatochromic Fluorescent Lipid Droplet Probe for Fatty Liver Disease Diagnosis

The prominent pathological feature of fatty liver disease lesions is excessive fat accumulation in lipid droplets in hepatocytes. Thus, developing fluorescent lipid droplet-specific probes with high permeability and a high imaging contrast provides a robust tool for diagnosing fatty liver diseases....

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Published inAnalytical chemistry (Washington) Vol. 94; no. 9; pp. 3881 - 3887
Main Authors Wu, Cheng-Juan, Li, Xin-Yu, Zhu, Ting, Zhao, Mengying, Song, Zhuoyue, Li, Shijie, Shan, Guo-Gang, Niu, Guangle
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 08.03.2022
Subjects
Accumulation
analytical chemistry
Animal models
Animals
Charge transfer
Chemistry
Diagnosis
disease diagnosis
disease models
Droplets
Emissions
Fatty liver
Fatty Liver - diagnostic imaging
fluorescence
Fluorescent Dyes
Fluorescent indicators
Hepatocytes
Lipid Droplets
Lipids
Lipophilic
lipophilicity
Liver
Liver diseases
Medical imaging
Mice
Microscopy, Fluorescence
Permeability
photostability
Polar environments
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Summary:The prominent pathological feature of fatty liver disease lesions is excessive fat accumulation in lipid droplets in hepatocytes. Thus, developing fluorescent lipid droplet-specific probes with high permeability and a high imaging contrast provides a robust tool for diagnosing fatty liver diseases. Herein, we rationally developed a novel donor–acceptor lipophilic fluorescent probe ANI with high photostability for wash-free visualization of lipid droplets and fatty liver disease characteristics. ANI showed a typical twisted intramolecular charge transfer effect with very faint fluorescence in high-polar solvents, but dramatically boosted emissions in low-polar environments. The solvatochromic probe can selectively light up lipid droplets with a high contrast in a wash-free manner. Further use of ANI to reveal the excessive accumulation of lipid droplets with a significantly large size in the liver tissues from the fatty liver disease model mice was successfully demonstrated. The remarkable imaging performances rendered ANI an alternative tool for accurately evaluating fatty liver disease in intraoperative diagnosis.
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ISSN:0003-2700
1520-6882
1520-6882
DOI:10.1021/acs.analchem.1c04847