Aerobic dehalogenation potentials of four bacterial species isolated from soil and sewage sludge

• Published in: Chemosphere (Oxford) Vol. 45; no. 1; pp. 45 - 50
• Main Authors: Olaniran, A.O, Babalola, G.O, Okoh, A.I
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.10.2001; Elsevier
• Subjects: Adaptations; Applied sciences; Bacillus; Bacillus - enzymology; Bacillus - isolation & purification; Bacillus - physiology; Bacillus sp; Bacteria, Aerobic - physiology; Bacterial aerobic dehalogenation; Biodegradation of pollutants; Biological and medical sciences; Biotechnology; Corynebacterium - enzymology; Corynebacterium - isolation & purification; Corynebacterium - physiology; Corynebacterium sp; Decontamination. Miscellaneous; Earth sciences; Earth, ocean, space; Engineering and environment geology. Geothermics; Environment and pollution; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Halogens - metabolism; Hydrogen-Ion Concentration; Industrial applications and implications. Economical aspects; Oxidation-Reduction; Pollution; Pollution, environment geology; Serial adaption; Sewage - microbiology; Soil and sediments pollution; Soil Microbiology; Soil Pollutants - metabolism; Nigeria; Africa; Serial adaption; Bacillus sp; Bacterial aerobic dehalogenation; Corynebacterium sp; Microbial activity; Aerobiosis; Chloroform; Dehalogenation; In situ; Carbon tetrachloride; Bacillaceae; Soil pollution; Sewage sludge; Corynebacterium; Bacillales; Enzymatic activity; Decontamination; Medium effect; Organochlorine compounds; Chlorine Organic compounds; Bacillus; Bacteria; Actinomycetes; Corynebacteriaceae; Bioremediation; Xenobiotic
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/S0045-6535(01)00075-3

Four bacterial species each were isolated from soil and a sewage oxidation pond using enrichment culture technique, and the bacterial isolates were identified to belong to the two genera Bacillus and Corynebacterium. The axenic cultures of the isolates utilized monochloroacetic acid (MCA), trichloroacetic acid (TCA), CHCl 3 and CCl 4 for growth up to 1 g substrate l −1 (w/v) and growths were enhanced in the mixed cultures of the isolates. The specific growth rate constants in the media ranged from 0.144 to 0.475. This is lower than the comparatively high values observed for the glucose medium, which varied significantly ( P<0.05) between 0.699 and 0.792 h −1 . Serial adaptation of the individual isolates in the organochloride media significantly ( P<0.05) affected the bacteria growth yields. The dehalogenase specific activity observed in the cell-free extracts of the mixed cultures of the isolates was significantly higher ( P<0.05) than those of their respective monocultures. Optimal pH of the dehalogenase activity was between 7.6 and 8.0, while temperature optima were between 30°C and 35°C.