Properties of Omp2a-Based Supported Lipid Bilayers: Comparison with Polymeric Bioinspired Membranes

• Published in: ACS omega Vol. 3; no. 8; pp. 9003 - 9019
• Main Authors: Puiggalí-Jou, Anna, Pawlowski, Jan, del Valle, Luis J, Michaux, Catherine, Perpète, Eric A, Sek, Slawomir, Alemán, Carlos
• Format: Journal Article Publication
• Language: English
• Published: United States American Chemical Society 31.08.2018; American Chemical Society (ACS)
• Subjects: Biological membrane; Biomedical materials; Enginyeria química; Materials biomèdics; Mechanical properties; Membranes (Technology); Membranes (Tecnologia); Nanostructures; Proteins; Proteïnes; Àrees temàtiques de la UPC
• ISSN: 2470-1343; 2470-1343
• DOI: 10.1021/acsomega.8b00913

Omp2a β-barrel outer membrane protein has been reconstituted into supported lipid bilayers (SLBs) to compare the nanomechanical properties (elastic modulus, adhesion forces, and deformation) and functionality of the resulting bioinspired system with those of Omp2a-based polymeric nanomembranes (NMs). Protein reconstitution into lipid bilayers has been performed using different strategies, the most successful one consisting of a detergent-mediated process into preformed liposomes. The elastic modulus obtained for the lipid bilayer and Omp2a are ∼19 and 10.5 ± 1.7 MPa, respectively. Accordingly, the protein is softer than the lipid bilayer, whereas the latter exhibits less mechanical strength than polymeric NMs. Besides, the function of Omp2a in the SLB is similar to that observed for Omp2a-based polymeric NMs. Results open the door to hybrid bioinspired substrates based on the integration of Omp2a-proteoliposomes and nanoperforated polymeric freestanding NMs.