Preparation Strategy for Uniformly Sized, Polymer-Based HPLC Packing Materials Having Practically Acceptable Column Efficiency. 1. Copolymerization Technique

• Published in: Analytical chemistry (Washington) Vol. 73; no. 24; pp. 5852 - 5857
• Main Authors: Hosoya, Ken, Teramachi, Masashi, Tanaka, Nobuo, Kobayashi, Atsushi, Kanda, Taketoshi, Ohtsu, Yutaka
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 15.12.2001
• Subjects: Biological and medical sciences; Chemistry; Fundamental and applied biological sciences. Psychology; Molecular and cellular biology; Polymers
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac0105991

Uniformly sized, polymer-based packing materials affording excellent chromatographic performance in semimicro HPLC were prepared from mixtures of alkyl methacrylate and glycerol dimethacrylate by a copolymerization technique using a multistep swelling and polymerization method. A column efficiency of up to 13 000 plates was obtained for a 2-mm-i.d. × 150-mm column with beads prepared from a 1:1 (v:v) mixture of an alkyl methacrylate and glycerol dimethacrylate. The packing materials exhibit a considerable improvement in column efficiency, as demonstrated on the separation of polyaromatic hydrocarbons (PAHs) having a relatively long retention time, which thus far has been a serious problem of polymer-based packing materials. Detailed studies suggested a rather limited optimum pore size, and its distribution was depicted to get the excellent column efficiency on polymer-based packing materials.