Microscale Nonreductive Release of O-Linked Glycans for Subsequent Analysis through MALDI Mass Spectrometry and Capillary Electrophoresis
A new β-elimination-based procedure has been devised for a microscale release of O-linked oligosaccharides from glycoproteins. Unlike the conventional Carlson degradation, which leads to formation of alditols, the procedure reported here renders the reducing end intact. Conversion of the liberated o...
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Published in | Analytical chemistry (Washington) Vol. 73; no. 24; pp. 6063 - 6069 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
American Chemical Society
15.12.2001
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Subjects | |
Online Access | Get full text |
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Summary: | A new β-elimination-based procedure has been devised for a microscale release of O-linked oligosaccharides from glycoproteins. Unlike the conventional Carlson degradation, which leads to formation of alditols, the procedure reported here renders the reducing end intact. Conversion of the liberated oligosaccharides to glycosylamines in ammonia medium is followed by the production of the reducing oligosaccharides through the addition of boric acid. The quantitatively generated oligosaccharides with the reducing end can subsequently be derivatized with a fluorophoric reagent for capillary electrophoresis or, alternatively, analyzed through MALDI mass spectrometry. The microscale version of these chemical steps permits us to investigate structurally O-linked oligosaccharides at very low levels. |
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Bibliography: | ark:/67375/TPS-DFQZ6HV8-H istex:CC3605057A122988D365717F4E4A03C2423D0AB7 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0003-2700 1520-6882 |
DOI: | 10.1021/ac015534c |