Generating Multiply Charged Protein Ions by Ultrasonic Nebulization/Multiple Channel-Electrospray Ionization Mass Spectrometry
An ultrasonic nebulization/multiple channel electrospray ionization (USN/MC-ES) source, which generates multiply charged peptides and proteins ions, was developed. The source is an ultrasonic nebulizer that is connected to a multiple channel electrospray ionization source. Aerosols were formed by ul...
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Published in | Analytical chemistry (Washington) Vol. 73; no. 20; pp. 4983 - 4987 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
American Chemical Society
15.10.2001
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Subjects | |
Online Access | Get full text |
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Summary: | An ultrasonic nebulization/multiple channel electrospray ionization (USN/MC-ES) source, which generates multiply charged peptides and proteins ions, was developed. The source is an ultrasonic nebulizer that is connected to a multiple channel electrospray ionization source. Aerosols were formed by ultrasonically nebulizing the sample solution. The aerosols were then purged into the central channel of a seven-channel ES source via nitrogen gas. A methanol solution that contained 1% trifluroacetic acid was electrosprayed through the outlying six electrosprayers. Detection of multiply charged peptide and protein ions indicated that electrospray was generated from the charged droplet containing analyte. The sample aerosol appeared to fuse with the charged methanol droplet in the air. Then electrospray ionization of the analyte occurred from the newly formed droplet. The peptide and protein prepared in deionized water were detected by this USN/MC-ES-MS. By varying the electrospray solvents, the signals of certain components in the mixture were selectively suppressed. |
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Bibliography: | istex:08E6705B9A87F1713D8E36AC49E48F3F707C6404 ark:/67375/TPS-SGCBHBHT-X ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0003-2700 1520-6882 |
DOI: | 10.1021/ac0101609 |