Microtubule Sensors and Sensor Array Based on Polyaniline Synthesized in the Presence of Poly(styrene sulfonate)

• Published in: Analytical chemistry (Washington) Vol. 75; no. 21; pp. 5673 - 5679
• Main Authors: Kanungo, Mandakini, Kumar, Anil, Contractor, A. Q
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.11.2003; AmericanChemical Society
• Subjects: Analysis; Biological and medical sciences; Biosensors; Biotechnology; Chemistry; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Sensors; Various methods and equipments; Performance evaluation; Polymer films; Glucose; Triglyceride; Optimization; Urea; Sensitivity; Response time; Biosensor; Styrenesulfonate polymer; Membrane; Microtubule; Sensor array; Polycarbonate; Immobilized enzyme; Aniline polymer
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac034537h

Microtubule sensors for glucose, urea, and triglyceride were fabricated based on poly(styrene sulfonate)−polyaniline (PSS−PANI) composites synthesized within the pores of track-etched polycarbonate membranes. The synthesis of a sufficiently thick and conducting PSS−PANI film at pH 5 provided the advantage of immobilizing enzymes during polymerization. This resulted in the improvement of sensor response for urea and triglyceride by a factor of ∼102 with a significant increase in the linear region of response compared to polyaniline-based sensors, where the enzymes were immobilized by physical adsorption after the polymerization. The sensors based on urea and triglyceride were found to have a higher linear range of response, better sensitivity, improved multiple use capability, and faster response time compared to the potentiometric and amperometric sensors based on polyaniline. A microtubule sensor array for glucose, urea, and triglyceride based on PSS−PANI was fabricated by immobilization of three different sets of enzymes on three closely spaced devices and its response was found to be free from cross-interference when a sample containing a mixture of the above analytes was analyzed in a single measurement.