Study on the Antiinflammatory Activity of Essential Oil from Leaves of Cinnamomum osmophloeum

• Published in: Journal of agricultural and food chemistry Vol. 53; no. 18; pp. 7274 - 7278
• Main Authors: Chao, Louis Kuoping, Hua, Kuo-Feng, Hsu, Hsien-Yeh, Cheng, Sen-Sung, Liu, Ju-Yun, Chang, Shang-Tzen
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 07.09.2005
• Subjects: Animals; anti-inflammatory activity; Anti-Inflammatory Agents - pharmacology; Aroma and flavouring agent industries; Biological and medical sciences; Cell Death - drug effects; Cell Line; Cinnamomum; Cinnamomum - chemistry; Cytokines - biosynthesis; essential oils; Food industries; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; interleukin-1; interleukin-6; leaves; Lipopolysaccharides - pharmacology; macrophages; Macrophages - drug effects; Macrophages - metabolism; Mice; monoterpenoids; Oils, Volatile - chemistry; Oils, Volatile - pharmacology; plant extracts; Plant Leaves - chemistry; protein synthesis; Terpenes - analysis; Cinnamomum osmophloeum; Antiinflammatory agent; Plant leaf; leaf; GC-MS; macrophages; Plant part; Dicotyledones; Angiospermae; Spermatophyta; Essential oil; cytokines; Cinnamomum; Lauraceae; antiinflammatory activity; essential oils
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf051151u

The leaf essential oil from indigenous cinnamon (Cinnamomum osmophloeum Kaneh.) was investigated by gas chromatography−mass spectrometry, and 21 compounds were identified. The major constituents of leaf essential oil were the monoterpenes 1,8-cineole (17.0%) and santolina triene (14.2%) and the sesquiterpenes spathulenol (15.7%) and caryophyllene oxide (11.2%). In the antiinflammatory activity assay, we demonstrated that the essential oil has a higher capacity to inhibit proIL-1β protein expression induced by LPS-treated J774A.1 murine macrophage. At dosages of 60 μg/mL, essential oil clearly inhibited proIL-1β protein expression. Furthermore, a dose of 60 μg/mL of essential oil was effectively inhibitory for IL-1β and IL-6 production but not for TNF-α, suggesting that essential oil was bioactive in antiinflammation in vitro. This study is the first to report antiinflammatory activity of extracts obtained from the leaf essential oil of C. osmophloeum. Keywords: Cinnamomum osmophloeum; leaf; essential oils; GC-MS; antiinflammatory activity; cytokines; macrophages