Biochemical screening of highly toxic aromatic contaminants in river sediment and comparison of sensitivity of biological model systems

• Published in: Chemosphere (Oxford) Vol. 30; no. 7; pp. 1363 - 1371
• Main Authors: Mádová, Lenka, Machala, Miroslav, Nezveda, Karel, Granátová, Marta, Nevoránková, Zdena
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.04.1995; Elsevier
• Subjects: Animal, plant and microbial ecology; Animals; Applied ecology; Benzofurans - analysis; Benzofurans - isolation & purification; Benzofurans - toxicity; Biochemical monitoring; Biological and medical sciences; Biological Assay; Carcinoma, Hepatocellular - pathology; Cell culture; Chick Embryo; Cytochrome P-450 CYP1A1; Cytochrome P-450 Enzyme System - biosynthesis; Cytochrome P450 1Al; Dibenzofurans, Polychlorinated; Ecotoxicology, biological effects of pollution; Environmental Monitoring; Enzyme Induction - drug effects; Fresh Water; Fresh water environment; Freshwater; Fundamental and applied biological sciences. Psychology; Hepa-1; Liver - drug effects; Liver - embryology; Liver - enzymology; Liver Neoplasms - pathology; Mice; Microsomes, Liver - drug effects; Microsomes, Liver - enzymology; Oxidoreductases - biosynthesis; Polychlorinated Biphenyls - analysis; Polychlorinated Biphenyls - isolation & purification; Polychlorinated Biphenyls - toxicity; Polychlorinated Dibenzodioxins - analogs & derivatives; Polychlorinated Dibenzodioxins - analysis; Polychlorinated Dibenzodioxins - isolation & purification; Polychlorinated Dibenzodioxins - toxicity; Polycyclic Compounds - analysis; Polycyclic Compounds - isolation & purification; Polycyclic Compounds - toxicity; Sensitivity and Specificity; Tumor Cells, Cultured; Water Pollutants, Chemical - analysis; Water Pollutants, Chemical - isolation & purification; Water Pollutants, Chemical - toxicity; Czech Rep., Dyje R; Chick embryo; Cell culture; Hepa-1; Cytochrome P450 1Al; Biochemical monitoring; Embryo; Ecotoxicology; Isozyme; Cytochrome P450; Fresh water; Rivers; Polycyclic aromatic compound; Sediments; Polychlorobiphenyl; In vitro; Pollutant; Investigation method; Test; Stream
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/0045-6535(95)00028-7

Fractions containing polycyclic aromatic hydrocarbons (PAHs), polychlorinated dibenzo-p-dioxins and dibenzofirrans (PCDD/Fs) and polychlorinated biphenyls (PCBs) were extracted from river sediments by various extraction methods. The amount of individual pollutants was determined analytically and data compared with biological assays. These were based on the induction of cytochrome P450 1Al (CYPIA1) after treatment with sediment fraction; in two different biological model systems, a mouse hepatoma cell line Hepa-1 and a chick embryo. In the hepatoma cell culture Hepa-1 significant correlations with analytical results were found for fractions containing PCDD/Fs and planar and mono-ortho-chlorinated PCBs. However for PAH fraction an undesirable decrease of P450 1Al induction was observed in higher concentrations of this fraction. This decrease was not observed in the chick embryo liver microsomes and biological responses towards the PAH fractions correlated with analytical data. Comparative investigations demonstrated that the chicken embryo hepatic microsomes were more sensitive for PAHs, and the hepatoma cell line Hepa-1 for PCDD/Fs and planar and mono-ortho-chlorinated PCBs.